UniProt Accession

 PININ_MOUSE; O35691; Q8CD89; Q8CGU3 

Genbank Protein ID

 Y08701; AY129403; AK030978 

Genbank Nucleotide ID

 CAA69960.1; AAN05017.1; BAC27200.1 

Protein Name

 Pinin 

Protein Synonyms/Alias

  

Gene Name

 Pnn 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
20	EKAKESLKNVDENIR	acetylation	[1]
136	QDQNMDEKGKQRNRR	ubiquitination	[2]
156	MGTLQKFKQESTVAT	acetylation	[1]
237	KYIRTKTKPHLFYIP	acetylation	[1, 3]
237	KYIRTKTKPHLFYIP	succinylation	[3]
253	RMCPATQKLIEESQR	acetylation	[1]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion (By similarity). 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 58 58 Phosphoserine (By similarity).
 MOD_RES 66 66 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 100 100 Phosphoserine.
 MOD_RES 114 114 Phosphoserine (By similarity).
 MOD_RES 115 115 Phosphoserine (By similarity).
 MOD_RES 237 237 N6-acetyllysine (By similarity).
 MOD_RES 346 346 Phosphoserine.
 MOD_RES 380 380 Phosphoserine.
 MOD_RES 440 440 Phosphoserine.
 MOD_RES 442 442 Phosphoserine.
 MOD_RES 666 666 Phosphoserine (By similarity).
 MOD_RES 679 679 Phosphoserine.
 MOD_RES 700 700 Phosphoserine (By similarity).
 MOD_RES 703 703 Phosphoserine (By similarity).  

Keyword

 Acetylation; Activator; Cell junction; Coiled coil; Complete proteome; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Spliceosome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 725 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0030057; C:desmosome; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR006786; Pinin_SDK_MemA.
 IPR006787; Pinin_SDK_N. 

Pfam

 PF04696; Pinin_SDK_memA
 PF04697; Pinin_SDK_N 

SMART

  

PROSITE

  

PRINTS