CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008572
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tricarboxylate transport protein, mitochondrial 
Protein Synonyms/Alias
 Citrate transport protein; CTP; Solute carrier family 25 member 1; Tricarboxylate carrier protein 
Gene Name
 SLC25A1 
Gene Synonyms/Alias
 SLC20A3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21APASGKAKLTHPGKAubiquitination[1]
97LLYGSIPKAAVRFGMacetylation[2, 3]
97LLYGSIPKAAVRFGMubiquitination[1, 4, 5]
160DQTSPNPKYRGFFHGacetylation[2]
160DQTSPNPKYRGFFHGubiquitination[4, 5]
178IVREQGLKGTYQGLTubiquitination[4, 5]
190GLTATVLKQGSNQAIubiquitination[4, 5, 6]
255MQGLEAHKYRNTWDCacetylation[2]
255MQGLEAHKYRNTWDCubiquitination[1, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in citrate-H(+)/malate exchange. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD(+) for the glycolytic pathway. 
Sequence Annotation
 REPEAT 23 111 Solcar 1.
 REPEAT 122 208 Solcar 2.
 REPEAT 218 303 Solcar 3.  
Keyword
 Complete proteome; Disease mutation; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Repeat; Transit peptide; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 311 AA 
Protein Sequence
MPAPRAPRAL AAAAPASGKA KLTHPGKAIL AGGLAGGIEI CITFPTEYVK TQLQLDERSH 60
PPRYRGIGDC VRQTVRSHGV LGLYRGLSSL LYGSIPKAAV RFGMFEFLSN HMRDAQGRLD 120
STRGLLCGLG AGVAEAVVVV CPMETIKVKF IHDQTSPNPK YRGFFHGVRE IVREQGLKGT 180
YQGLTATVLK QGSNQAIRFF VMTSLRNWYR GDNPNKPMNP LITGVFGAIA GAASVFGNTP 240
LDVIKTRMQG LEAHKYRNTW DCGLQILKKE GLKAFYKGTV PRLGRVCLDV AIVFVIYDEV 300
VKLLNKVWKT D 311 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; TAS:UniProtKB.
 GO:0015137; F:citrate transmembrane transporter activity; TAS:UniProtKB.
 GO:0006094; P:gluconeogenesis; TAS:Reactome.
 GO:0035338; P:long-chain fatty-acyl-CoA biosynthetic process; TAS:Reactome.
 GO:0006843; P:mitochondrial citrate transport; TAS:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0019432; P:triglyceride biosynthetic process; TAS:Reactome. 
Interpro
 IPR002067; Mit_carrier.
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 
Pfam
 PF00153; Mito_carr 
SMART
  
PROSITE
 PS50920; SOLCAR 
PRINTS
 PR00926; MITOCARRIER.