CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001828
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tryptophan biosynthesis protein TrpCF 
Protein Synonyms/Alias
 Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI 
Gene Name
 trpC 
Gene Synonyms/Alias
 b1262; JW1254 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
7*MQTVLAKIVADKAIacetylation[1]
55TAFILECKKASPSKGacetylation[1]
56AFILECKKASPSKGVacetylation[1]
61CKKASPSKGVIRDDFacetylation[1]
78ARIAAIYKHYASAISacetylation[1]
179RAIALGAKVVGINNRacetylation[1]
204RTRELAPKLGHNVTVacetylation[1]
258RVLLGENKVCGLTRGacetylation[1]
322DIADVVDKAKVLSLAacetylation[1]
375REFQHVDKYVLDNGQacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the TrpF domain; the second reaction is catalyzed by the synthase, coded by the TrpC domain. 
Sequence Annotation
 REGION 1 256 Indole-3-glycerol phosphate synthase.
 REGION 257 452 N-(5'-phosphoribosyl)anthranilate  
Keyword
 3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Complete proteome; Decarboxylase; Isomerase; Lyase; Multifunctional enzyme; Reference proteome; Tryptophan biosynthesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MQTVLAKIVA DKAIWVEARK QQQPLASFQN EVQPSTRHFY DALQGARTAF ILECKKASPS 60
KGVIRDDFDP ARIAAIYKHY ASAISVLTDE KYFQGSFNFL PIVSQIAPQP ILCKDFIIDP 120
YQIYLARYYQ ADACLLMLSV LDDDQYRQLA AVAHSLEMGV LTEVSNEEEQ ERAIALGAKV 180
VGINNRDLRD LSIDLNRTRE LAPKLGHNVT VISESGINTY AQVRELSHFA NGFLIGSALM 240
AHDDLHAAVR RVLLGENKVC GLTRGQDAKA AYDAGAIYGG LIFVATSPRC VNVEQAQEVM 300
AAAPLQYVGV FRNHDIADVV DKAKVLSLAA VQLHGNEEQL YIDTLREALP AHVAIWKALS 360
VGETLPAREF QHVDKYVLDN GQGGSGQRFD WSLLNGQSLG NVLLAGGLGA DNCVEAAQTG 420
CAGLDFNSAV ESQPGIKDAR LLASVFQTLR AY 452 
Gene Ontology
 GO:0004425; F:indole-3-glycerol-phosphate synthase activity; IDA:EcoCyc.
 GO:0004640; F:phosphoribosylanthranilate isomerase activity; IDA:EcoCyc.
 GO:0000162; P:tryptophan biosynthetic process; IMP:EcoCyc. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR013798; Indole-3-glycerol_P_synth.
 IPR001468; Indole-3-GlycerolPSynthase_CS.
 IPR001240; PRAI_dom.
 IPR011060; RibuloseP-bd_barrel. 
Pfam
 PF00218; IGPS
 PF00697; PRAI 
SMART
  
PROSITE
 PS00614; IGPS 
PRINTS