UniProt Accession

 RNC_ECOLI; P0A7Y0; P05797; P06141; Q2MAG3 

Genbank Protein ID

 X02946; X02673; D64044; U36841; U00096; AP009048; M26415; M14658 

Genbank Nucleotide ID

 CAA26692.1; CAA26504.1; BAA10914.1; AAA79829.1; AAC75620.1; BAE76743.1; AAA21843.1; AAA03241.1 

Protein Name

 Ribonuclease 3 

Protein Synonyms/Alias

 Ribonuclease III; RNase III 

Gene Name

 rnc 

Gene Synonyms/Alias

 b2567; JW2551 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
102	RLGPGELKSGGFRRE	acetylation	[1]
152	DEISPGDKQKDPKTR	acetylation	[1]
210	GTGSSRRKAEQAAAE	acetylation	[1, 2]
221	AAAEQALKKLELE**	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Digests double-stranded RNA formed within single-strand substrates, but not RNA-DNA hybrids. Involved in the processing of rRNA precursors, viral transcripts, some mRNAs and at least 1 tRNA (metY, a minor form of tRNA-init-Met). Cleaves the 30S primary rRNA transcript to yield the immediate precursors to the 16S and 23S rRNAs; cleavage can occur in assembled 30S, 50S and even 70S subunits and is influenced by the presence of ribosomal proteins. The E.coli enzyme does not cleave R.capsulatus rRNA precursor, although R.capsulatus will complement an E.coli disruption, showing substrate recognition is different. Removes the intervening sequences from Salmonella typhimurium rRNA precursor. 

Sequence Annotation

 DOMAIN 6 128 RNase III.
 DOMAIN 155 225 DRBM.
 ACT_SITE 45 45 Potential.
 ACT_SITE 117 117 Probable.
 METAL 41 41 Magnesium (Probable).
 METAL 114 114 Magnesium (By similarity).
 METAL 117 117 Magnesium (Probable).  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Endonuclease; Hydrolase; Magnesium; Metal-binding; mRNA processing; Nuclease; Nucleotide-binding; Reference proteome; RNA-binding; rRNA processing; rRNA-binding; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 226 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004525; F:ribonuclease III activity; IDA:EcoCyc.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:HAMAP.
 GO:0006396; P:RNA processing; IDA:EcoCyc.
 GO:0016075; P:rRNA catabolic process; IEA:InterPro.
 GO:0006364; P:rRNA processing; IEA:HAMAP.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR011907; RNase_III.
 IPR000999; RNase_III_dom. 

Pfam

 PF00035; dsrm
 PF00636; Ribonuclease_3 

SMART

 SM00358; DSRM
 SM00535; RIBOc 

PROSITE

 PS50137; DS_RBD
 PS00517; RNASE_3_1
 PS50142; RNASE_3_2 

PRINTS