CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023629
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nck-associated protein 1 
Protein Synonyms/Alias
 NAP 1; Membrane-associated protein HEM-2; p125Nap1 
Gene Name
 NCKAP1 
Gene Synonyms/Alias
 HEM2; KIAA0587; NAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12VLQPSQQKLAEKLTIubiquitination[1]
16SQQKLAEKLTILNDRubiquitination[1, 2]
58KNLESAVKFIVRKFPubiquitination[2]
81QQLAQLQKEKSEILKubiquitination[1]
190VDYENPLKKMMEEFVubiquitination[1]
351KFLRSALKELATVLSubiquitination[1]
501QAYTSVSKASLGLADubiquitination[1]
895DQMAALFKRLSSVDSubiquitination[2]
1075KIGQETDKTTTRNREubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cell membrane; Cell projection; Complete proteome; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1128 AA 
Protein Sequence
MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI 60
VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDVCQVF 120
FDITVNFDLT KNYLDLIITY TTLMILLSRI EERKAIIGLY NYAHEMTHGA SDREYPRLGQ 180
MIVDYENPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML 240
NPAQSDTMPC EYLSLDAMEK WIIFGFILCH GILNTDATAL NLWKLALQSS SCLSLFRDEV 300
FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL KELATVLSDQ 360
PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF IDKHIAELIF YMEELRAHVR 420
KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV 480
FDFRGMRLDW FRLQAYTSVS KASLGLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS 540
IFCFYSRAFE KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN 600
MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP 660
GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMVVWEH TFTPREYLTS HLEIRFTKSI 720
VGMTMYNQAT QEIAKPSELL TSVRAYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG 780
EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS 840
ELLGPYGMKF LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV 900
DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET DMKVAMNVYE 960
LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM VFVAVSLPTL ASNVMSQYSP 1020
AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN 1080
RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVTSSA 1128 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
 GO:0045176; P:apical protein localization; IEA:Compara.
 GO:0006915; P:apoptotic process; TAS:ProtInc.
 GO:0045175; P:basal protein localization; IEA:Compara.
 GO:0007417; P:central nervous system development; TAS:ProtInc.
 GO:0010172; P:embryonic body morphogenesis; IEA:Compara.
 GO:0048617; P:embryonic foregut morphogenesis; IEA:Compara.
 GO:0035050; P:embryonic heart tube development; IEA:Compara.
 GO:0007492; P:endoderm development; IEA:Compara.
 GO:0030950; P:establishment or maintenance of actin cytoskeleton polarity; IEA:Compara.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0008078; P:mesodermal cell migration; IEA:Compara.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0048570; P:notochord morphogenesis; IEA:Compara.
 GO:0048340; P:paraxial mesoderm morphogenesis; IEA:Compara.
 GO:0050821; P:protein stabilization; IEA:Compara.
 GO:0032880; P:regulation of protein localization; IEA:Compara.
 GO:0001756; P:somitogenesis; IEA:Compara.
 GO:0007354; P:zygotic determination of anterior/posterior axis, embryo; IEA:Compara. 
Interpro
 IPR019137; Nck-associated_protein-1. 
Pfam
 PF09735; Nckap1 
SMART
  
PROSITE
  
PRINTS