CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014427
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription elongation factor SPT6 
Protein Synonyms/Alias
  
Gene Name
 Supt6h 
Gene Synonyms/Alias
 Kiaa0162 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
538GLDGLAKKFGLTPEQubiquitination[1]
777FMDENQGKGIRVLGIubiquitination[1]
844KKFLVNKKPHVVTIAubiquitination[1]
1100LENPERLKDLDLDAFubiquitination[1]
1676HAAIDWGKMAEQWLQacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Acts to stimulate transcriptional elongation by RNA polymerase II (By similarity). 
Sequence Annotation
 DOMAIN 1213 1282 S1 motif.
 DOMAIN 1325 1431 SH2.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 7 7 Phosphoserine (By similarity).
 MOD_RES 12 12 Phosphoserine (By similarity).
 MOD_RES 73 73 Phosphoserine (By similarity).
 MOD_RES 75 75 Phosphoserine (By similarity).
 MOD_RES 78 78 Phosphoserine (By similarity).
 MOD_RES 125 125 Phosphoserine.
 MOD_RES 267 267 Phosphoserine (By similarity).
 MOD_RES 743 743 N6-acetyllysine (By similarity).
 MOD_RES 1523 1523 Phosphothreonine (By similarity).
 MOD_RES 1532 1532 Phosphothreonine (By similarity).
 MOD_RES 1535 1535 Phosphoserine (By similarity).
 MOD_RES 1539 1539 Phosphothreonine (By similarity).
 MOD_RES 1676 1676 N6-acetyllysine (By similarity).
 MOD_RES 1697 1697 Phosphothreonine (By similarity).
 MOD_RES 1701 1701 Phosphoserine (By similarity).
 MOD_RES 1703 1703 Phosphoserine (By similarity).
 MOD_RES 1718 1718 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1726 AA 
Protein Sequence
MSDFVESEAE ESEEEYNHEG EVVPRVTKKF VEEEDDDEEE EEENLDDQDE RGNLKDFIND 60
DDDEEEGEED EGSDSGDSED DVGHKKRKRP SFDDRLEDDD FDLIEENLGV KVKRGQKYRR 120
VKKMSDDDED DEEEYGKEEH EKEAIAGEIF QDEEGEEGQE AVEAPMAPPD EEEEDDEESD 180
IDDFIVDDDG QPLKKPKWRK KLPGYTDAAL QEAQEIFGVD FDYDEFEKYN EYDEELEEDY 240
EYEDDETEGE IRVRPKKTTK KRVSRRSIFE MYEPSELESS HLTDQDNEIR ATDLPERFQL 300
RSIPVKAAED DELEEEADWI YRNAFATPTI SLQDSCDYLD RGQPTSSFSR KGPSTVQKIK 360
EALGFMRNQH FEVPFIAFYR KEYVEPELHI NDLWRVWQWD EKWTQLRIRK ENLTRLFEKM 420
QAYQYEQISA DPDKPLADGI RALDTTDMER LKDVQSMDEL KDVYNHFLLY YGRDIPKMQN 480
AAKASRKKLK RIKEDGDEEG EGEEAEDEEQ RGPELKQASR RDMYTICQSA GLDGLAKKFG 540
LTPEQFGENL RDSYQRHETE QFPAEPLELA KDYVCSQFPT PEAVLEGARY MVALQIAREP 600
LVRQVLRQTF QERAKLNITP TKKGRKDVDE AHYAYSFKYL KNKPVKELRD DQFLKIGLAE 660
DEGLLTIDIS IDMKGVEGYG NDQTYFEEIK QFYYRDEFSH QVQEWNRQRT MAIERALQQF 720
LYVQMAKELK NKLLAEARES VVKACSRKLY NWLRVAPYRP DQQVEEDDDF MDENQGKGIR 780
VLGIAFSSAR DHPVFCALVN GEGEVTDFLR LPHFTKRRTA WREEEREKKA QDIETLKKFL 840
VNKKPHVVTI AGENRDAQML TEDVKRIVHE LDQGQQLSSI GVELVDNELA ILYMNSKKSE 900
AEFRDYPPVL RQAVSLARRI QDPLIEFAQV CSSDEDILCL KFHPLQEHVV KEELLNALYC 960
EFINRVNEVG VDVNRAIAHP YSQALIQYVC GLGPRKGTHL LKILKQNNTR LESRTQLVTM 1020
CHMGPKVFMN CAGFLKIDTA SLGDSTDSYI EVLDGSRVHP ETYEWARKMA VDALEYDESA 1080
EDANPAGALE EILENPERLK DLDLDAFAEE LERQGYGDKH ITLYDIRAEL SCRYKDLRTA 1140
YRSPNTEEIF NMLTKETPET FYIGKLIICN VTGIAHRRPQ GESYDQAIRN DETGLWQCPF 1200
CQQDNFPELS EVWNHFDSGS CPGQAIGVKT RLDNGVTGFI PTKFLSDKVV KRPEERVKVG 1260
MTVHCRIMKI DIEKFSADLT CRTSDLMDRN NEWKLPKDTY YDFDAEAADH KQEEDMKRKQ 1320
QRTTYIKRVI AHPSFHNINF KQAEKMMETM DQGDVIIRPS SKGENHLTVT WKVSAGIYQH 1380
VDVREEGKEN AFSLGATLWI NSEEFEDLDE IVARYVQPMA SFARDLLNHK YYQDCSGGDR 1440
KKLEELLIKT KKEKPTFIPY FICACKELPG KFLLGYQPRG KPRIEYVTVT PEGFRYRGQI 1500
FPTVNGLFRW FKDHYQDPVP GITPSSSNRT RTPASINATP ANINLADLTR AVNALPQNMT 1560
SQMFSAIAAV TGQGQNPNAT PAQWASSQYG YGGSGGGSSA YHVFPTPAQQ PVATPLMTPS 1620
YSYTTPSQPI TTPQYHQLQA STTPQSTQAQ PQPSSSSRQR QQQPKSNSHA AIDWGKMAEQ 1680
WLQEKEAERR KQKQRLTPRP SPSPMIESTP MSIAGDATPL LDEMDR 1726 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0032784; P:regulation of DNA-dependent transcription, elongation; IEA:InterPro.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR012340; NA-bd_OB-fold.
 IPR003029; Rbsml_prot_S1_RNA-bd_dom.
 IPR022967; RNA-binding_domain_S1.
 IPR000980; SH2.
 IPR023323; Tex-like_dom.
 IPR023097; Tex_RuvX-like_dom.
 IPR017072; TF_Spt6.
 IPR006641; YqgF/RNaseH-like_dom. 
Pfam
 PF00575; S1
 PF00017; SH2 
SMART
 SM00316; S1
 SM00252; SH2
 SM00732; YqgFc 
PROSITE
 PS50126; S1
 PS50001; SH2 
PRINTS