CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000950
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional protein NCOAT 
Protein Synonyms/Alias
 Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; Protein O-GlcNAcase; Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA; Histone acetyltransferase; HAT 
Gene Name
 MGEA5 
Gene Synonyms/Alias
 HEXC; KIAA0679; MEA5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87ELFRRLQKWELNTYLubiquitination[1, 2]
98NTYLYAPKDDYKHRMubiquitination[3]
157EVSTLKRKLDQVSQFubiquitination[3, 4]
240YLRTVGEKLLPGIEVubiquitination[1, 2, 3, 5]
253EVLWTGPKVVSKEIPubiquitination[3]
257TGPKVVSKEIPVESIubiquitination[3, 5]
269ESIEEVSKIIKRAPVubiquitination[3]
289HANDYDQKRLFLGPYubiquitination[3]
297RLFLGPYKGRSTELIubiquitination[1, 2, 3, 5]
597SVVSVNCKGKDSEKIubiquitination[3]
599VSVNCKGKDSEKIEEubiquitination[3]
613EWRSRAAKFEEMCGLubiquitination[3]
713PPLTPTSKVYTIRPYubiquitination[3]
723TIRPYFPKDEASVYKubiquitination[1, 2, 3, 6]
730KDEASVYKICREMYDubiquitination[3, 6]
794TPFIKKCKISWIPFMubiquitination[3]
807FMQEKYTKPNGDKELubiquitination[3]
858VTDPSVAKSMMACLLubiquitination[3]
869ACLLSSLKANGSRGAubiquitination[3]
885CEVRPDDKRILEFYSubiquitination[3]
893RILEFYSKLGCFEIAubiquitination[3]
901LGCFEIAKMEGFPKDubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 
Sequence Annotation
 REGION 583 916 Histone acetyltransferase activity (By
 REGION 695 814 Required for histone H4 binding (By
 ACT_SITE 175 175 Nucleophile; for O-GlcNAcase activity (By
 ACT_SITE 177 177 Proton donor; for O-GlcNAcase activity
 MOD_RES 364 364 Phosphoserine.
 DISULFID 777 793 By similarity.  
Keyword
 Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Disulfide bond; Glycosidase; Hydrolase; Multifunctional enzyme; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 916 AA 
Protein Sequence
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV AGAAGGARRF 60
LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD YKHRMFWREM YSVEEAEQLM 120
TLISAAREYE IEFIYAISPG LDITFSNPKE VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM 180
CAADKEVFSS FAHAQVSITN EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK 240
LLPGIEVLWT GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 300
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED STVSIQIKLE 360
NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR QVAHSGAKAS VVDGTPLVAA 420
PSLNATTVVT TVYQEPIMSQ GAALSGEPTT LTKEEEKKQP DEEPMDMVVE KQEETDHKND 480
NQILSEIVEA KMAEELKPMD TDKESIAESK SPEMSMQEDC ISDIAPMQTD EQTNKEQFVP 540
GPNEKPLYTA EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD 600
SEKIEEWRSR AAKFEEMCGL VMGMFTRLSN CANRTILYDM YSYVWDIKSI MSMVKSFVQW 660
LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND LFFQPPPLTP TSKVYTIRPY 720
FPKDEASVYK ICREMYDDGV GLPFQSQPDL IGDKLVGGLL SLSLDYCFVL EDEDGICGYA 780
LGTVDVTPFI KKCKISWIPF MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP 840
SLIKMDIHKK VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA 900
KMEGFPKDVV ILGRSL 916 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0004415; F:hyalurononglucosaminidase activity; TAS:ProtInc.
 GO:0007568; P:aging; IEA:Compara.
 GO:0046060; P:dATP metabolic process; IEA:Compara.
 GO:0006516; P:glycoprotein catabolic process; TAS:ProtInc.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:Compara.
 GO:0070265; P:necrotic cell death; IEA:Compara.
 GO:0010616; P:negative regulation of cardiac muscle adaptation; IEA:Compara.
 GO:0060051; P:negative regulation of protein glycosylation; IEA:Compara.
 GO:0010524; P:positive regulation of calcium ion transport into cytosol; IEA:Compara.
 GO:0031343; P:positive regulation of cell killing; IEA:Compara.
 GO:0051054; P:positive regulation of DNA metabolic process; IEA:Compara.
 GO:0046326; P:positive regulation of glucose import; IEA:Compara.
 GO:0060124; P:positive regulation of growth hormone secretion; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0051901; P:positive regulation of mitochondrial depolarization; IEA:Compara.
 GO:0043243; P:positive regulation of protein complex disassembly; IEA:Compara.
 GO:0045862; P:positive regulation of proteolysis; IEA:Compara.
 GO:0006612; P:protein targeting to membrane; IEA:Compara.
 GO:0048545; P:response to steroid hormone stimulus; IEA:Compara. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR011496; Beta-N-acetylglucosaminidase.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF07555; NAGidase 
SMART
  
PROSITE
  
PRINTS