UniProt Accession

 CMPK2_HUMAN; Q5EBM0; A2RUB0; A5D8T2; B7ZM18; Q6ZRU2; Q96AL8 

Genbank Protein ID

 AK127983; BC016969; BC141802; BC132821; BC089425; BC144202 

Genbank Nucleotide ID

 BAC87217.1; AAH16969.1; AAI41803.1; AAI32822.1; AAH89425.1; AAI44203.1 

Protein Name

 UMP-CMP kinase 2, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 CMPK2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
245	DLVDQCPKQIQKGKF	ubiquitination	[1, 2]
251	PKQIQKGKFQVVAIE	ubiquitination	[1]
265	EGLDATGKTTVTQSV	ubiquitination	[1]
277	QSVADSLKAVLLKSP	ubiquitination	[1, 3]
282	SLKAVLLKSPPSCIG	ubiquitination	[1, 3]
293	SCIGQWRKIFDDEPT	ubiquitination	[1]
320	IVASEIAKESAKSPV	ubiquitination	[1]
324	EIAKESAKSPVIVDR	ubiquitination	[1]
393	LQGRGMEKTREEAEL	ubiquitination	[1]
409	ANSVFRQKVEMSYQR	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. May be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs. 

Sequence Annotation

 NP_BIND 259 266 ATP (Potential).  

Keyword

 Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Kinase; Mitochondrion; Nucleotide-binding; Polymorphism; Pyrimidine biosynthesis; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 449 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004127; F:cytidylate kinase activity; IDA:MGI.
 GO:0004798; F:thymidylate kinase activity; IEA:Compara.
 GO:0033862; F:UMP kinase activity; IDA:MGI.
 GO:0071222; P:cellular response to lipopolysaccharide; IEA:Compara.
 GO:0006233; P:dTDP biosynthetic process; IEA:Compara.
 GO:0006227; P:dUDP biosynthetic process; IEA:Compara. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR014505; UMP-CMP_kinase_mit. 

Pfam

  

SMART

  

PROSITE

 PS01331; THYMIDYLATE_KINASE 

PRINTS