UniProt Accession

 CBPS_YEAST; P27614; D6VW16 

Genbank Protein ID

 X57316; X63068; Z49447; BK006943 

Genbank Nucleotide ID

 CAA40571.1; CAA44790.1; CAA89467.1; DAA08632.1 

Protein Name

 Carboxypeptidase S 

Protein Synonyms/Alias

 GLY-X carboxypeptidase; YSCS 

Gene Name

 CPS1 

Gene Synonyms/Alias

 CPS; YJL172W; J0510 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
8	MIALPVEKAPRKSLW	ubiquitination	[1, 2, 3]
374	FCPRRKDKLVEYISN	acetylation	[4]
442	YAKKIAEKYGYGLSK	acetylation	[4]

Reference

 [1] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [4] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Necessary for use of certain peptides as sole nitrogen source. May also cleave intracellularly generated peptides to recycle amino acids for protein synthesis. 

Sequence Annotation

 ACT_SITE 170 170 By similarity.
 ACT_SITE 239 239 Proton acceptor (By similarity).
 METAL 168 168 Zinc 2 (By similarity).
 METAL 205 205 Zinc 1 (By similarity).
 METAL 205 205 Zinc 2 (By similarity).
 METAL 240 240 Zinc 1 (By similarity).
 METAL 268 268 Zinc 2 (By similarity).
 METAL 547 547 Zinc 1 (By similarity).
 CARBOHYD 88 88 N-linked (GlcNAc...) (Potential).
 CARBOHYD 176 176 N-linked (GlcNAc...) (Potential).
 CARBOHYD 228 228 N-linked (GlcNAc...) (Potential).
 CARBOHYD 381 381 N-linked (GlcNAc...) (Potential).
 CARBOHYD 525 525 N-linked (GlcNAc...) (Potential).  

Keyword

 Carboxypeptidase; Complete proteome; Glycoprotein; Hydrolase; Membrane; Metal-binding; Protease; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation; Vacuole; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 576 AA 

Protein Sequence

Gene Ontology

 GO:0000328; C:fungal-type vacuole lumen; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0004180; F:carboxypeptidase activity; IMP:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
 GO:0006807; P:nitrogen compound metabolic process; IMP:SGD.
 GO:0051603; P:proteolysis involved in cellular protein catabolic process; IMP:SGD. 

Interpro

 IPR001261; ArgE/DapE_CS.
 IPR017141; Pept_M20_carboxypep.
 IPR002933; Peptidase_M20.
 IPR011650; Peptidase_M20_dimer. 

Pfam

 PF07687; M20_dimer
 PF01546; Peptidase_M20 

SMART

  

PROSITE

 PS00758; ARGE_DAPE_CPG2_1
 PS00759; ARGE_DAPE_CPG2_2 

PRINTS