CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006567
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-3 
Protein Synonyms/Alias
 Actin-dependent myosin-I MYO3; Class I unconventional myosin MYO3; Type I myosin MYO3 
Gene Name
 MYO3 
Gene Synonyms/Alias
 YKL129C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
135SGESGAGKTEAAKRIubiquitination[1]
1048SSAIPAAKVSSKHSSacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 One of two redundant type-I myosins implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization and for the internalization step in endocytosis. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions redundantly with LAS17 as actin nucleation-promoting factor (NPF) for the Arp2/3 complex. Motor domain phosphorylation by PAK kinases CLA4 and STE20 promotes CDC42-regulated actin assembly. Functions together with the NPF PAN1 in late stages of endocytosis. Motor domain phosphorylation by PDK1 kinases PKH1 and PKH2, and by SGK kinases YPK1 and YPK2, promotes ligand-induced, but not constitutive endocytosis of the G protein-coupled receptor STE2. 
Sequence Annotation
 DOMAIN 38 701 Myosin head-like.
 DOMAIN 719 739 IQ 1.
 DOMAIN 740 765 IQ 2.
 DOMAIN 1120 1182 SH3.
 NP_BIND 129 136 ATP (Potential).
 REGION 404 486 Actin-binding (By similarity).
 REGION 759 961 Basic, putative membrane-binding region.
 MOD_RES 357 357 Phosphoserine.  
Keyword
 3D-structure; Actin-binding; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1272 AA 
Protein Sequence
MAVIKKGARR KDVKEPKKRS AKIKKATFDA NKKKEVGISD LTLLSKISDE SINENLKKRF 60
KNGIIYTYIG HVLISVNPFR DLGIYTNAVL ESYKGKNRLE VPPHVFAIAE SMYYNLKSYN 120
ENQCVIISGE SGAGKTEAAK RIMQYIAAAS NSHSESIGKI KDMVLATNPL LESFGCAKTL 180
RNNNSSRHGK YLEIKFNSQF EPCAGNITNY LLEKQRVVGQ IKNERNFHIF YQFTKGASDT 240
YKQMFGVQMP EQYIYTAAAG CTTADTIDDV KDYEGTLEAM RTIGLVQEEQ DQIFRMLAAI 300
LWIGNISFIE NEEGNAQVGD TSVTDFVAYL LQVDASLLVK CLVERIMQTS HGMKRGSVYH 360
VPLNPVQATA VRDALAKAIY NNLFDWIVDR VNVSLQAFPG ADKSIGILDI YGFEIFEHNS 420
FEQICINYVN EKLQQIFIQL TLKAEQETYE REKIKWTPIK YFDNKVVCDL IEAKNPPGIL 480
AAMNDSIATA HADSNAADQA FAQRLNLFNS NPYFELRANK FVIKHYAGDV TYDINGITDK 540
NKDQLQKDLI ELIGTTTNTF LSTIFPDDVD KDSKRRPPTA GDKIIKSANE LVETLSKAEP 600
SYIRTIKPNQ TKSPNDYDDH QVLHQVKYLG LQENVRIRRA GFAYRQTFEK FVERFYLLSP 660
DCSYAGDYTW DGDTLEAVKL ILRDAMIPEK EFQLGVTSVF IKTPESLFAL EDMRDKYWYN 720
MAARIQRAWR RFLQRRIDAA IKIQRTIREK KGGNKYVKLR DYGTKLLAGK KERRSMSLLG 780
YRAFMGDYLS CNESKTKGSY IRRQVGIKDK VVFSIKGECL HSKFGRSAQR LKKVFILTKK 840
TFYIIGQTRE QNAMKYTQDY KIDVGKIKQV SLTNLQDDWM GVILVNSTQS DPLINTPFKT 900
ELMTRLKKLN EKIMIKVGPT IEYHKQPNKL HTVRSKISDS APKYGDIYKS STIYVRRGHP 960
ANSKSNKKPK NPGGLSGKPI KSKKSKHKST HKHTHSHRSH RDAAKKQPLP SQKPVNPLSL 1020
AATAAQAAYN PKPDKTVPIK SSAIPAAKVS SKHSSKPSSK EKVAVKKASS SHKSSSAKQN 1080
QVSMPPSKGV EKNKEPLKET TATATANIPI PPPPPPMGQP KDPKFEAAYD FPGSGSSSEL 1140
PLKKGDIVFI SRDEPSGWSL AKLLDGSKEG WVPTAYMTPY KDTRNTVPVA ATGAVNDVTN 1200
QKSSQIDNTI SSAQEGVQFG SATVGPTSDN QSNPVGTFSD GLASALAARA NKMRAESADD 1260
DDNDDGDDDD DW 1272 
Gene Ontology
 GO:0030479; C:actin cortical patch; TAS:SGD.
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000146; F:microfilament motor activity; TAS:SGD.
 GO:0051666; P:actin cortical patch localization; IGI:SGD.
 GO:0007121; P:bipolar cellular bud site selection; TAS:SGD.
 GO:0006897; P:endocytosis; TAS:SGD.
 GO:0006887; P:exocytosis; TAS:SGD.
 GO:0031505; P:fungal-type cell wall organization; TAS:SGD.
 GO:0006970; P:response to osmotic stress; TAS:SGD. 
Interpro
 IPR001609; Myosin_head_motor_dom.
 IPR010926; Myosin_tail_2.
 IPR027417; P-loop_NTPase.
 IPR001452; SH3_domain. 
Pfam
 PF00063; Myosin_head
 PF06017; Myosin_TH1
 PF00018; SH3_1 
SMART
 SM00242; MYSc
 SM00326; SH3 
PROSITE
 PS50096; IQ
 PS50002; SH3 
PRINTS
 PR00193; MYOSINHEAVY.