CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022567
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 42 
Protein Synonyms/Alias
 Cullin-3-binding protein 9; Ctb9; Kelch domain-containing protein 5 
Gene Name
 KLHL42 
Gene Synonyms/Alias
 KIAA1340; KLHDC5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20DRCYPVSKRKLIEQSubiquitination[1]
22CYPVSKRKLIEQSDYubiquitination[1, 2]
290QVASMNQKRSNFKLVubiquitination[1, 2]
502SSLYLPNKAET****ubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL42) E3 ubiquitin ligase complex mediates the ubiquitination and subsequent degradation of KATNA1. Involved in microtubule dynamics throughout mitosis. 
Sequence Annotation
 DOMAIN 5 78 BTB.
 REPEAT 176 234 Kelch 1.
 REPEAT 235 282 Kelch 2.
 REPEAT 284 325 Kelch 3.
 REPEAT 327 372 Kelch 4.
 REPEAT 374 429 Kelch 5.
 REPEAT 431 480 Kelch 6.
 MOD_RES 43 43 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kelch repeat; Mitosis; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MSAEEMVQIR LEDRCYPVSK RKLIEQSDYF RALYRSGMRE ALSQEAGGPE VQQLRGLSAP 60
GLRLVLDFIN AGGAREGWLL GPRGEKGGGV DEDEEMDEVS LLSELVEAAS FLQVTSLLQL 120
LLSQVRLNNC LEMYRLAQVY GLPDLQEACL RFMVVHFHEV LCKPQFHLLG SPPQAPGDVS 180
LKQRLREARM TGTPVLVALG DFLGGPLAPH PYQGEPPSML RYEEMTERWF PLANNLPPDL 240
VNVRGYGSAI LDNYLFIVGG YRITSQEISA AHSYNPSTNE WLQVASMNQK RSNFKLVAVN 300
SKLYAIGGQA VSNVECYNPE QDAWNFVAPL PNPLAEFSAC ECKGKIYVIG GYTTRDRNMN 360
ILQYCPSSDM WTLFETCDVH IRKQQMVSVE ETIYIVGGCL HELGPNRRSS QSEDMLTVQS 420
YNTVTRQWLY LKENTSKSGL NLTCALHNDG IYIMSRDVTL STSLEHRVFL KYNIFSDSWE 480
AFRRFPAFGH NLLVSSLYLP NKAET 505 
Gene Ontology
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0000209; P:protein polyubiquitination; IDA:UniProtKB.
 GO:0032886; P:regulation of microtubule-based process; IMP:UniProtKB. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF01344; Kelch_1 
SMART
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS