UniProt Accession

 NSUN4_HUMAN; Q96CB9; A8K6S6; B3KQ50; B4DHA4; Q5TDF7; Q96AN8; Q9HAJ8 

Genbank Protein ID

 AK021577; AK057420; AK291741; AK295003; AL122001; CH471059; BC014441; BC016907 

Genbank Nucleotide ID

 BAB13847.1; BAG51912.1; BAF84430.1; BAG58065.1; CAI21962.1; EAX06917.1; AAH14441.1; AAH16907.1 

Protein Name

 5-methylcytosine rRNA methyltransferase NSUN4 

Protein Synonyms/Alias

 NOL1/NOP2/Sun domain family member 4 

Gene Name

 NSUN4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
37	KWAATEPKFPAVRLA	ubiquitination	[1, 2]
215	SRIARLQKILHSYVP	ubiquitination	[1, 2, 3]
240	VTSWDGRKWGELEGD	ubiquitination	[1, 2]
274	EEENNIFKRSRKKER	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Involved in mitochondrial ribosome large subunit biogenesis. 

Sequence Annotation

 REGION 181 187 S-adenosyl-L-methionine binding.
 ACT_SITE 310 310 Nucleophile.
 BINDING 237 237 S-adenosyl-L-methionine.
 BINDING 255 255 S-adenosyl-L-methionine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Methyltransferase; Mitochondrion; Polymorphism; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 384 AA 

Protein Sequence

Gene Ontology

 GO:0005762; C:mitochondrial large ribosomal subunit; IDA:MGI.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR001678; Fmu/NOL1/Nop2p.
 IPR023267; RCMT. 

Pfam

 PF01189; Nol1_Nop2_Fmu 

SMART

  

PROSITE

 PS01153; NOL1_NOP2_SUN 

PRINTS

 PR02008; RCMTFAMILY.