| Tag | Content |
|---|
| CPLM ID | CPLM-018123 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 25 |
Protein Synonyms/Alias | Mediator complex subunit 25; mMED25 |
Gene Name | Med25 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 274 | ALRLLFEKAAPPALL | ubiquitination | [1] | | 561 | NKDLESLKGLYRIMG | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity). |
Sequence Annotation | REGION 1 226 Interaction with the Mediator complex (By
REGION 389 543 Interaction with VP16 (By similarity).
REGION 395 545 Interaction with CREBBP (By similarity).
REGION 563 652 Interaction with RARA (By similarity).
REGION 639 705 Interaction with RARA (By similarity).
MOTIF 645 649 LXXLL motif. |
Keyword | Activator; Alternative splicing; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 745 AA |
Protein Sequence | MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG PPAETDFGGD 60
YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKPVLGAEPY PDPARMSNSL 120
SLLPANGIPR KLSSGIVKCR CLLRTSGRNP HLEFWEAPRP SLLHIRDGAY EDSTTFGQMF 180
MGGGGESCSL IAEGLSTALQ LFDDFKKMRE QIGQTHRVCL LICNSPPYLL PAVESTTYSG 240
CTTESLVQKI GERGIHFSIV SPRKLPALRL LFEKAAPPAL LEPLQQPADV SQDPRHMVLV 300
RGLVLPVGGS STSGSLQTKQ AVPLPPAPAS AATLSAAPPQ ALPPVPPQYQ VPGNLSAAQV 360
AAQNAVEAAK SQKAGLGPRF SPINPLQQAA PGVGPPFSQA PAPPLAPVPP GAPKPPPASQ 420
PSLVSTVAPG PVLAAPAQPG APSLAGTVTP GGVNGPSAAQ LGGPALGGQQ SVSNKLLAWS 480
GVLEWQEKPK PASVDANTKL TRSLPCQVYV NHGENLKTEQ WPQKLIMQLI PQQLLTTLGP 540
LFRNSRMVQF HFTNKDLESL KGLYRIMGNG FAGCVHFPHT APCEVRVLML LYSSKKKIFM 600
GLIPYDQSGF VNGIRQVITN HKQVQQQKLE QQRGMGAQQA PPVLGPILEE QARPPQNLLQ 660
LRAPQPQPQG AVGASAATGQ PQPQGATQAP TGAPQGPPGA APGPPPSGPI LRPQNPGANP 720
QLRSLLLNPA PPQTGVPPPQ ASLHHLQPPG APTLLPPHQS MGQPQLGPQL LHPPPAQSWP 780
TQLPQRAPLP GQMLLSGGPR GPVPQPGLQP SVMEDDILMD LI 822 |
Gene Ontology | GO:0005654; C:nucleoplasm; TAS:Reactome. GO:0048147; P:negative regulation of fibroblast proliferation; IEA:Compara. GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara. GO:0071158; P:positive regulation of cell cycle arrest; IEA:Compara. GO:0035563; P:positive regulation of chromatin binding; IEA:Compara. GO:2001178; P:positive regulation of mediator complex assembly; IEA:Compara. GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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