CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023227
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Synergin gamma 
Protein Synonyms/Alias
 AP1 subunit gamma-binding protein 1; Gamma-synergin 
Gene Name
 SYNRG 
Gene Synonyms/Alias
 AP1GBP1; SYNG 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
513KALPSMDKYAVFKGIacetylation[1]
744NSTAASTKYDVFRQLacetylation[1, 2]
907DDATQGRKLSPFVLSubiquitination[3]
1104RSNTLNEKPALPVIRubiquitination[3]
1113ALPVIRDKYKDLTGEubiquitination[3]
1115PVIRDKYKDLTGEVEubiquitination[3]
1144SALNVIKKANDTLNGubiquitination[3]
1197ATAVCSEKLQQLLKDubiquitination[4]
1271PAEEHPKKAFNSETDubiquitination[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins. 
Sequence Annotation
 DOMAIN 295 388 EH.
 REGION 518 786 Interaction with A1P1G1 and A1P1G2.
 MOTIF 457 461 DFXDF motif 1.
 MOTIF 690 694 DFXDF motif 2.
 MOTIF 775 779 DFXDF motif 3.
 MOD_RES 473 473 Phosphoserine.
 MOD_RES 513 513 N6-acetyllysine.
 MOD_RES 720 720 Phosphoserine.
 MOD_RES 744 744 N6-acetyllysine.
 MOD_RES 752 752 Phosphoserine.
 MOD_RES 852 852 Phosphoserine.
 MOD_RES 855 855 Phosphoserine.
 MOD_RES 909 909 Phosphoserine.
 MOD_RES 919 919 Phosphoserine.
 MOD_RES 935 935 Phosphoserine.
 MOD_RES 1075 1075 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Endocytosis; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1314 AA 
Protein Sequence
MALRPGAGSG GGGAAGAGAG SAGGGGFMFP VAGGIRPPQA GLMPMQQQGF PMVSVMQPNM 60
QGIMGMNYSS QMSQGPIAMQ AGIPMGPMPA AGMPYLGQAP FLGMRPPGPQ YTPDMQKQFA 120
EEQQKRFEQQ QKLLEEERKR RQFEEQKQKL RLLSSVKPKT GEKSRDDALE AIKGNLDGFS 180
RDAKMHPTPA SHPKKPGPSL EEKFLVSCDI STSGQEQIKL NTSEVGHKAL GPGSSKKYPS 240
LMASNGVAVD GCVSGTTTAE AENTSDQNLS IEESGVGVFP SQDPAQPRMP PWIYNESLVP 300
DAYKKILETT MTPTGIDTAK LYPILMSSGL PRETLGQIWA LANRTTPGKL TKEELYTVLA 360
MIAVTQRGVP AMSPDALNQF PAAPIPTLSG FSMTLPTPVS QPTVIPSGPA GSMPLSLGQP 420
VMGINLVGPV GGAAAQASSG FIPTYPANQV VKPEEDDFQD FQDASKSGSL DDSFSDFQEL 480
PASSKTSNSQ HGNSAPSLLM PLPGTKALPS MDKYAVFKGI AADKSSENTV PPGDPGDKYS 540
AFRELEQTAE NKPLGESFAE FRSAGTDDGF TDFKTADSVS PLEPPTKDKT FPPSFPSGTI 600
QQKQQTQVKN PLNLADLDMF SSVNCSSEKP LSFSAVFSTS KSVSTPQSTG SAATMTALAA 660
TKTSSLADDF GEFSLFGEYS GLAPVGEQDD FADFMAFSNS SISSEQKPDD KYDALKEEAS 720
PVPLTSNVGS TVKGGQNSTA ASTKYDVFRQ LSLEGSGLGV EDLKDNTPSG KSDDDFADFH 780
SSKFSSINSD KSLGEKAVAF RHTKEDSASV KSLDLPSIGG SSVGKEDSED ALSVQFDMKL 840
ADVGGDLKHV MSDSSLDLPT VSGQHPPAAD IEDLKYAAFG SYSSNFAVST LTSYDWSDRD 900
DATQGRKLSP FVLSAGSGSP SATSILQKKE TSFGSSENIT MTSLSKVTTF VSEDALPETT 960
FPALASFKDT IPQTSEQKEY ENRDYKDFTK QDLPTAERSQ EATCPSPASS GASQETPNEC 1020
SDDFGEFQSE KPKISKFDFL VATSQSKMKS SEEMIKSELA TFDLSVQGSH KRSLSLGDKE 1080
ISRSSPSPAL EQPFRDRSNT LNEKPALPVI RDKYKDLTGE VEENERYAYE WQRCLGSALN 1140
VIKKANDTLN GISSSSVCTE VIQSAQGMEY LLGVVEVYRV TKRVELGIKA TAVCSEKLQQ 1200
LLKDIDKVWN NLIGFMSLAT LTPDENSLDF SSCMLRPGIK NAQELACGVC LLNVDSRSRK 1260
EEKPAEEHPK KAFNSETDSF KLAYGGHQYH ASCANFWINC VEPKPPGLVL PDLL 1314 
Gene Ontology
 GO:0030121; C:AP-1 adaptor complex; TAS:ProtInc.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006886; P:intracellular protein transport; TAS:ProtInc. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR000261; EPS15_homology. 
Pfam
  
SMART
  
PROSITE
 PS50031; EH 
PRINTS