CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000852
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 7 
Protein Synonyms/Alias
 Beta-Pix; PAK-interacting exchange factor beta 
Gene Name
 Arhgef7 
Gene Synonyms/Alias
 Pak3bp; Pixb 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
68REIKPSEKPVSPKSGacetylation[1]
357TTGMTITKLEDSENHacetylation[1]
627RARKDLEKLVRKVLKacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity). 
Sequence Annotation
 DOMAIN 6 65 SH3.
 DOMAIN 93 273 DH.
 DOMAIN 295 400 PH.
 MOD_RES 7 7 Phosphoserine (By similarity).
 MOD_RES 71 71 Phosphoserine (By similarity).
 MOD_RES 340 340 Phosphoserine (By similarity).
 MOD_RES 516 516 Phosphoserine; by CaMK1 (By similarity).
 MOD_RES 560 560 Phosphoserine (By similarity).  
Keyword
 3D-structure; Cell junction; Cell projection; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Neurogenesis; Phosphoprotein; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 646 AA 
Protein Sequence
MTDNANSQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTHNG RTGWFPSNYV 60
REIKPSEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN ILETEHEYSK ELQSVLSTYL 120
WPLQTSEKLS SANTSYLMGN LEEISSFQQV LVQSLEECTK SPEAQQRVGG CFLSLMPQMR 180
TLYLAYCANH PSAVSVLTEH SEDLGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK 240
ELERHMEDYH PDRQDIQKSM TAFKNLSAQC QEVRKRKELE LQILTEPIRS WEGDDIKTLG 300
SVTYMSQVTI QCAGSEEKNE RYLLLFPNLL LMLSASPRMS GFIYQGKLPT TGMTITKLED 360
SENHRNAFEI SGSMIERILV SCNNQQDLHE WVEHLQRQTK VTSVSNPTIK PHSVPSHTLP 420
SHPLTPSSKH ADSKPVALTP AYHTLPHPSH HGTPHTTISW GPLEPPKTPK PWSLSCLRPA 480
PPLRPSAALC YKEDLSRSPK TMKKLLPKRK PERKPSDEEF AVRKSTAALE EDAQILKVIE 540
AYCTSAKTRQ TLNSSSRKES APQVLLPEEE KIIVEETKSN GQTVIEEKSL VDTVYALKDE 600
VQELRQDNKK MKKSLEEEQR ARKDLEKLVR KVLKNMNDPA WDETNL 646 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:RGD.
 GO:0005925; C:focal adhesion; IDA:RGD.
 GO:0030426; C:growth cone; IDA:RGD.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; TAS:RGD.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0043615; P:astrocyte cell migration; IMP:RGD.
 GO:0030032; P:lamellipodium assembly; ISS:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IDA:RGD. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00169; PH
 PF00621; RhoGEF
 PF07653; SH3_2 
SMART
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.