CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014695
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Endoplasmin 
Protein Synonyms/Alias
 94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1 
Gene Name
 Hsp90b1 
Gene Synonyms/Alias
 Grp94; Tra1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
95LIINSLYKNKEIFLRacetylation[1]
142KIKCDREKNLLHVTDacetylation[1]
265YLELDTIKNLVRKYSacetylation[1]
356EVEEDEYKAFYKSFSacetylation[1]
404LFDEYGSKKSDYIKLacetylation[1]
410SKKSDYIKLYVRRVFacetylation[1]
455RETLQQHKLLKVIRKacetylation[1]
473RKTLDMIKKIADEKYacetylation[1]
479IKKIADEKYNDTFWKacetylation[1]
534YVERMKEKQDKIYFMacetylation[1]
537RMKEKQDKIYFMAGSacetylation[1]
597NVAKEGVKFDESEKSacetylation[1]
613ESREATEKEFEPLLNacetylation[1]
628WMKDKALKDKIEKAVacetylation[1]
630KDKALKDKIEKAVVSacetylation[1]
633ALKDKIEKAVVSQRLacetylation[1]
671AQAYQTGKDISTNYYacetylation[1]
682TNYYASQKKTFEINPacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity). 
Sequence Annotation
 MOTIF 801 804 Prevents secretion from ER.
 BINDING 107 107 ATP (By similarity).
 BINDING 149 149 ATP (By similarity).
 BINDING 162 162 ATP (By similarity).
 BINDING 168 168 ATP (By similarity).
 BINDING 199 199 ATP; via amide nitrogen (By similarity).
 BINDING 448 448 ATP (By similarity).
 CARBOHYD 62 62 N-linked (GlcNAc...) (Potential).
 CARBOHYD 107 107 N-linked (GlcNAc...) (Potential).
 CARBOHYD 217 217 N-linked (GlcNAc...) (Potential).
 CARBOHYD 445 445 N-linked (GlcNAc...) (Potential).
 CARBOHYD 481 481 N-linked (GlcNAc...) (Potential).
 CARBOHYD 502 502 N-linked (GlcNAc...) (Potential).
 DISULFID 138 138 Interchain (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Calcium; Chaperone; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Nucleotide-binding; Phosphoprotein; Reference proteome; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 804 AA 
Protein Sequence
MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ REEEAIQLDG 60
LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF LRELISNASD ALDKIRLISL 120
TDENALAGNE ELTVKIKCDR EKNLLHVTDT GVGMTREELV KNLGTIAKSG TSEFLNKMTE 180
AQEDGQSTSE LIGQFGVGFY SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT 240
LGRGTTITLV LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDETAQ 300
EEKEEADDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV EEDEYKAFYK 360
SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE YGSKKSDYIK LYVRRVFITD 420
DFHDMMPKYL NFVKGVVDSD DLPLNVSRET LQQHKLLKVI RKKLVRKTLD MIKKIADEKY 480
NDTFWKEFGT NIKLGVIEDH SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF 540
MAGSSRKEAE SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE 600
SEKSKESREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS QYGWSGNMER 660
IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR VKEDEDDKTV MDLAVVLFET 720
ATLRSGYLLP DTKAYGDRIE RMLRLSLNID PEAQVEEEPE EEPEDTTEDT TDDSEQDEEE 780
TDAGAEEEEE EQETEKEPTE KDEL 804 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:Compara.
 GO:0046790; F:virion binding; IEA:Compara.
 GO:0031247; P:actin rod assembly; IEA:Compara.
 GO:0071318; P:cellular response to ATP; IEA:Compara.
 GO:0030433; P:ER-associated protein catabolic process; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0043666; P:regulation of phosphoprotein phosphatase activity; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara. 
Interpro
 IPR015566; Endoplasmin.
 IPR003594; HATPase_ATP-bd.
 IPR019805; Heat_shock_protein_90_CS.
 IPR001404; Hsp90.
 IPR020575; Hsp90_N.
 IPR020568; Ribosomal_S5_D2-typ_fold. 
Pfam
 PF00183; HSP90 
SMART
 SM00387; HATPase_c 
PROSITE
 PS00014; ER_TARGET
 PS00298; HSP90 
PRINTS
 PR00775; HEATSHOCK90.