CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003654
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transposon Ty1-DR6 Gag-Pol polyprotein 
Protein Synonyms/Alias
 Gag-Pol-p199; TY1A-TY1B; Transposon Ty1 TYA-TYB polyprotein; p190; Capsid protein; CA; Gag-p45; p54; Ty1 protease; PR; Pol-p20; p23; Integrase; IN; Pol-p71; p84; p90; Reverse transcriptase/ribonuclease H; RT; RT-RH; Pol-p63; p60 
Gene Name
 TY1B-DR6 
Gene Synonyms/Alias
 YDRWTy1-5 POL; YDR365W-B; D9481.12 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
368RRNLSDEKNDSRSYTacetylation[1]
368RRNLSDEKNDSRSYTubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty1 RNA and initiation of reverse transcription (By similarity). 
Sequence Annotation
 DOMAIN 660 835 Integrase catalytic.
 DOMAIN 1338 1476 Reverse transcriptase Ty1/copia-type.
 DOMAIN 1610 1752 RNase H Ty1/copia-type.
 REGION 299 401 RNA-binding (By similarity).
 REGION 583 640 Integrase-type zinc finger-like.
 MOTIF 1178 1212 Bipartite nuclear localization signal (By
 ACT_SITE 461 461 For protease activity; shared with
 METAL 671 671 Magnesium; catalytic; for integrase
 METAL 736 736 Magnesium; catalytic; for integrase
 METAL 1346 1346 Magnesium; catalytic; for reverse
 METAL 1427 1427 Magnesium; catalytic; for reverse
 METAL 1428 1428 Magnesium; catalytic; for reverse
 METAL 1610 1610 Magnesium; catalytic; for RNase H
 METAL 1652 1652 Magnesium; catalytic; for RNase H
 METAL 1685 1685 Magnesium; catalytic; for RNase H
 MOD_RES 416 416 Phosphoserine (By similarity).  
Keyword
 Aspartyl protease; ATP-binding; Capsid maturation; Complete proteome; Cytoplasm; DNA integration; DNA recombination; DNA-binding; DNA-directed DNA polymerase; Endonuclease; Hydrolase; Magnesium; Metal-binding; Multifunctional enzyme; Nuclease; Nucleotide-binding; Nucleotidyltransferase; Nucleus; Phosphoprotein; Protease; Reference proteome; Ribosomal frameshifting; RNA-binding; RNA-directed DNA polymerase; Transferase; Transposable element; Transposition; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1755 AA 
Protein Sequence
MESQQLSQHS PISHGSACAS VTSKEVHTNQ DPLDVSASKT EECEKASTKA NSQQTTTPAS 60
SAVPENPHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG WSFYGHPSMI PYTPYQMSPM 120
YFPPGPQSQF PQYPSSVGTP LSTPSPESGN TFTDSSSADS DMTSTKKYVR PPPMLTSPND 180
FPNWVKTYIK FLQNSNLGGI IPTVNGKPVR QITDDELTFL YNTFQIFAPS QFLPTWVKDI 240
LSVDYTDIMK ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN 300
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ QGSRNSKPNY 360
RRNLSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA HNVSTSNNSP STDNDSISKS 420
TTEPIQLNNK HDLHLGQELT ESTVNHTNHS DDELPGHLLL DSGASRTLIR SAHHIHSASS 480
NPDINVVDAQ KRNIPINAIG DLQFHFQDNT KTSIKVLHTP NIAYDLLSLN ELAAVDITAC 540
FTKNVLERSD GTVLAPIVQY GDFYWVSKRY LLPSNISVPT INNVHTSEST RKYPYPFIHR 600
MLAHANAQTI RYSLKNNTIT YFNESDVDWS SAIDYQCPDC LIGKSTKHRH IKGSRLKYQN 660
SYEPFQYLHT DIFGPVHNLP NSAPSYFISF TDETTKFRWV YPLHDRREDS ILDVFTTILA 720
FIKNQFQASV LVIQMDRGSE YTNRTLHKFL EKNGITPCYT TTADSRAHGV AERLNRTLLD 780
DCRTQLQCSG LPNYLWFSAI EFSTIVRNSL ASPKSKKSAR QHAGLAGLDI STLLPFGQPV 840
IVNDHNPNSK IHPRGIPGYA LHPSRNSYGY IIYLPSLKKT VDTTNYVILQ GKESRLDQFN 900
YDALTFDEDL NRLTASYHSF IASNEIQESN DLNIESDHDF QSDIELHPEQ PRNVLSKAVS 960
PTDSTPPSTH TEDSKRVSKT NIRAPREVDP NISESNILPS KKRSSTPQIS NIESTGSGGM 1020
HKLNVPLLAP MSQSNTHESS HASKSKDFRH SDSYSENETN HTNVPISSTG GTNNKTVPQI 1080
SDQETEKRII HRSPSIDASP PENNSSHNIV PIKTPTTVSE QNTEESIIAD LPLPDLPPES 1140
PTEFPDPFKE LPPINSRQTN SSLGGIGDSN AYTTINSKKR SLEDNETEIK VSRDTWNTKN 1200
MRSLEPPRSK KRIHLIAAVK AVKSIKPIRT TLRYDEAITY NKDIKEKEKY IEAYHKEVNQ 1260
LLKMKTWDTD EYYDRKEIDP KRVINSMFIF NKKRDGTHKA RFVARGDIQH PDTYDSGMQS 1320
NTVHHYALMT SLSLALDNNY YITQLDISSA YLYADIKEEL YIRPPPHLGM NDKLIRLKKS 1380
LYGLKQSGAN WYETIKSYLI QQCGMEEVRG WSCVFKNSQV TICLFVDDMV LFSKNLNSNK 1440
RIIEKLKMQY DTKIINLGES DEEIQYDILG LEIKYQRGKY MKLGMENSLT EKIPKLNVPL 1500
NPKGRKLSAP GQPGLYIDQD ELEIDEDEYK EKVHEMQKLI GLASYVGYKF RFDLLYYINT 1560
LAQHILFPSR QVLDMTYELI QFMWDTRDKQ LIWHKNKPTK PDNKLVAISD ASYGNQPYYK 1620
SQIGNIFLLN GKVIGGKSTK ASLTCTSTTE AEIHAVSEAI PLLNNLSHLV QELNKKPIIK 1680
GLLTDSRSTI SIIKSTNEEK FRNRFFGTKA MRLRDEVSGN NLYVYYIETK KNIADVMTKP 1740
LPIKTFKLLT NKWIH 1755 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000943; C:retrotransposon nucleocapsid; ISS:SGD.
 GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003887; F:DNA-directed DNA polymerase activity; ISS:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008233; F:peptidase activity; ISS:SGD.
 GO:0004540; F:ribonuclease activity; ISS:SGD.
 GO:0004523; F:ribonuclease H activity; IEA:EC.
 GO:0003723; F:RNA binding; ISS:SGD.
 GO:0003964; F:RNA-directed DNA polymerase activity; ISS:SGD.
 GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0032197; P:transposition, RNA-mediated; ISS:SGD.
 GO:0046797; P:viral procapsid maturation; IEA:UniProtKB-KW. 
Interpro
 IPR001584; Integrase_cat-core.
 IPR001969; Peptidase_aspartic_AS.
 IPR015820; Retrotransposon_Ty1A_N.
 IPR012337; RNaseH-like_dom.
 IPR013103; RVT_2. 
Pfam
 PF00665; rve
 PF07727; RVT_2
 PF01021; TYA 
SMART
  
PROSITE
 PS00141; ASP_PROTEASE
 PS50994; INTEGRASE 
PRINTS