CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012088
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-binding protein Ikaros 
Protein Synonyms/Alias
 Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1 
Gene Name
 IKZF1 
Gene Synonyms/Alias
 IK1; IKAROS; LYF1; ZNFN1A1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58RVVASNVKVETQSDEsumoylation[1]
91MLDASGEKMNGSHRDubiquitination[2, 3]
157CGASFTQKGNLLRHIubiquitination[2, 3]
165GNLLRHIKLHSGEKPubiquitination[2]
171IKLHSGEKPFKCHLCubiquitination[2]
174HSGEKPFKCHLCNYAubiquitination[2]
241GTLYPVIKEETNHSEsumoylation[1]
281RKSSMPQKFLGDKGLubiquitination[3]
286PQKFLGDKGLSDTPYubiquitination[2, 3]
301DSSASYEKENEMMKSubiquitination[2, 3]
378ENLLLLSKAKLVPSEubiquitination[2, 3, 4]
380LLLLSKAKLVPSEREubiquitination[2, 3]
429ARNGLSLKEEHRAYDubiquitination[2, 3]
460STSGEQMKVYKCEHCubiquitination[2, 3]
Reference
 [1] Ikaros SUMOylation: switching out of repression.
 Gómez-del Arco P, Koipally J, Georgopoulos K.
 Mol Cell Biol. 2005 Apr;25(7):2688-97. [PMID: 15767674]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). 
Sequence Annotation
 ZN_FING 117 139 C2H2-type 1.
 ZN_FING 145 167 C2H2-type 2.
 ZN_FING 173 195 C2H2-type 3.
 ZN_FING 201 224 C2H2-type 4.
 ZN_FING 462 484 C2H2-type 5.
 ZN_FING 490 514 C2H2-type 6.
 REGION 154 163 Required for both high-affinity DNA
 REGION 180 195 Required for both high-affinity DNA
 REGION 468 471 Required for binding PP1CC (By
 MOD_RES 13 13 Phosphoserine (By similarity).
 MOD_RES 23 23 Phosphothreonine (By similarity).
 MOD_RES 63 63 Phosphoserine.
 MOD_RES 101 101 Phosphoserine (By similarity).
 MOD_RES 168 168 Phosphoserine (By similarity).
 MOD_RES 196 196 Phosphoserine (By similarity).
 MOD_RES 258 258 Phosphoserine.
 MOD_RES 261 261 Phosphoserine.
 MOD_RES 296 296 Phosphoserine (By similarity).
 MOD_RES 361 361 Phosphoserine.
 MOD_RES 364 364 Phosphoserine.
 MOD_RES 389 389 Phosphoserine (By similarity).
 MOD_RES 391 391 Phosphoserine (By similarity).
 MOD_RES 393 393 Phosphoserine (By similarity).
 MOD_RES 397 397 Phosphoserine (By similarity).
 MOD_RES 398 398 Phosphothreonine (By similarity).
 CROSSLNK 58 58 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 241 241 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Alternative splicing; Cell cycle; Chromatin regulator; Complete proteome; Cytoplasm; Developmental protein; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 519 AA 
Protein Sequence
MDADEGQDMS QVSGKESPPV SDTPDEGDEP MPIPEDLSTT SGGQQSSKSD RVVASNVKVE 60
TQSDEENGRA CEMNGEECAE DLRMLDASGE KMNGSHRDQG SSALSGVGGI RLPNGKLKCD 120
ICGIICIGPN VLMVHKRSHT GERPFQCNQC GASFTQKGNL LRHIKLHSGE KPFKCHLCNY 180
ACRRRDALTG HLRTHSVGKP HKCGYCGRSY KQRSSLEEHK ERCHNYLESM GLPGTLYPVI 240
KEETNHSEMA EDLCKIGSER SLVLDRLASN VAKRKSSMPQ KFLGDKGLSD TPYDSSASYE 300
KENEMMKSHV MDQAINNAIN YLGAESLRPL VQTPPGGSEV VPVISPMYQL HKPLAEGTPR 360
SNHSAQDSAV ENLLLLSKAK LVPSEREASP SNSCQDSTDT ESNNEEQRSG LIYLTNHIAP 420
HARNGLSLKE EHRAYDLLRA ASENSQDALR VVSTSGEQMK VYKCEHCRVL FLDHVMYTIH 480
MGCHGFRDPF ECNMCGYHSQ DRYEFSSHIT RGEHRFHMS 519 
Gene Ontology
 GO:0005721; C:centromeric heterochromatin; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030183; P:B cell differentiation; IEA:Compara.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0048732; P:gland development; IEA:Compara.
 GO:0048535; P:lymph node development; IEA:Compara.
 GO:0007498; P:mesoderm development; TAS:ProtInc.
 GO:0001779; P:natural killer cell differentiation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0048541; P:Peyer's patch development; IEA:Compara.
 GO:0040018; P:positive regulation of multicellular organism growth; IEA:Compara.
 GO:0045660; P:positive regulation of neutrophil differentiation; IEA:Compara.
 GO:0051138; P:positive regulation of NK T cell differentiation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0060041; P:retina development in camera-type eye; IEA:Compara.
 GO:0030217; P:T cell differentiation; IEA:Compara.
 GO:0048538; P:thymus development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS