| Tag | Content |
|---|
| CPLM ID | CPLM-002061 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Partitioning protein REP1 |
Protein Synonyms/Alias | R1; Protein Baker; Trans-acting factor B |
Gene Name | REP1 |
Gene Synonyms/Alias | R0020C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 146 | ESPFVNTKVTAFFSY | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4- containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. |
Sequence Annotation | REGION 1 129 Interaction with REP2 and self-
REGION 1 76 Interaction with REP2.
REGION 349 373 Nuclear localization. |
Keyword | Complete proteome; Nucleus; Plasmid; Plasmid partition; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 373 AA |
Protein Sequence | MNGERLLACI KQCIMQHFQP MVYDESRCVI ETTRGTFPVP DNYKKYKTLA FAFVGHVLNT 60
DDTPVIEKEL DWPDPALVYN TIVDRIINHP ELSQFISVAF ISQLKATIGE GLDINVKGTL 120
NRRGKGIRRP KGVFFRYMES PFVNTKVTAF FSYLRDYNKI ASEYHNNTKF ILTFSCQAYW 180
ASGPNFSALK NVIRCSIIHE YISKFVEREQ DKGHIGDQEL PPEEDPSREL NNVQHEVNSL 240
TEQDAEADEG LWGEIDSLCE KWQSEAEDQT EAEIIADRII GNSQRMANLK IRRTKFKSVL 300
YHILKELIQS QGTVKVYRGS SFSHDSIKIS LHYEEQHITA VWVYLTVKFE EHWKPVDVEV 360
EFRCKFKERK VDG 373 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |