CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007086
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 
Protein Synonyms/Alias
 Oligosaccharyl transferase subunit STT3 
Gene Name
 STT3 
Gene Synonyms/Alias
 YGL022W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
56WFNYRATKYLVNNSFacetylation[1]
56WFNYRATKYLVNNSFubiquitination[2]
437DFKTSDRKYAIKPAAubiquitination[2]
489PSQTPDGKLALIDDFubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. 
Sequence Annotation
 CARBOHYD 60 60 N-linked (GlcNAc...) (Potential).
 CARBOHYD 535 535 N-linked (GlcNAc...) (Potential).
 CARBOHYD 539 539 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; Complete proteome; Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Membrane; Reference proteome; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 718 AA 
Protein Sequence
MGSDRSCVLS VFQTILKLVI FVAIFGAAIS SRLFAVIKFE SIIHEFDPWF NYRATKYLVN 60
NSFYKFLNWF DDRTWYPLGR VTGGTLYPGL MTTSAFIWHA LRNWLGLPID IRNVCVLFAP 120
LFSGVTAWAT YEFTKEIKDA SAGLLAAGFI AIVPGYISRS VAGSYDNEAI AITLLMVTFM 180
FWIKAQKTGS IMHATCAALF YFYMVSAWGG YVFITNLIPL HVFLLILMGR YSSKLYSAYT 240
TWYAIGTVAS MQIPFVGFLP IRSNDHMAAL GVFGLIQIVA FGDFVKGQIS TAKFKVIMMV 300
SLFLILVLGV VGLSALTYMG LIAPWTGRFY SLWDTNYAKI HIPIIASVSE HQPVSWPAFF 360
FDTHFLIWLF PAGVFLLFLD LKDEHVFVIA YSVLCSYFAG VMVRLMLTLT PVICVSAAVA 420
LSKIFDIYLD FKTSDRKYAI KPAALLAKLI VSGSFIFYLY LFVFHSTWVT RTAYSSPSVV 480
LPSQTPDGKL ALIDDFREAY YWLRMNSDED SKVAAWWDYG YQIGGMADRT TLVDNNTWNN 540
THIAIVGKAM ASPEEKSYEI LKEHDVDYVL VIFGGLIGFG GDDINKFLWM IRISEGIWPE 600
EIKERDFYTA EGEYRVDARA SETMRNSLLY KMSYKDFPQL FNGGQATDRV RQQMITPLDV 660
PPLDYFDEVF TSENWMVRIY QLKKDDAQGR TLRDVGELTR SSTKTRRSIK RPELGLRV 718 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0008250; C:oligosaccharyltransferase complex; IPI:SGD.
 GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IPI:SGD.
 GO:0006487; P:protein N-linked glycosylation; IPI:SGD. 
Interpro
 IPR003674; Oligo_trans_STT3. 
Pfam
 PF02516; STT3 
SMART
  
PROSITE
  
PRINTS