CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017039
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium homeostasis endoplasmic reticulum protein 
Protein Synonyms/Alias
 ERPROT 213-21; SR-related CTD-associated factor 6 
Gene Name
 CHERP 
Gene Synonyms/Alias
 DAN26; SCAF6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18ELRNVIDKLAQFVARacetylation[1]
18ELRNVIDKLAQFVARubiquitination[2, 3]
177KDAISAGKNWMFSNAubiquitination[2, 3, 4, 5, 6, 7]
879KGGDVRDKWDQYKGVacetylation[1]
879KGGDVRDKWDQYKGVubiquitination[2, 3]
901YENYRRNKSYSFIARacetylation[1]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Involved in calcium homeostasis, growth and proliferation. 
Sequence Annotation
 REPEAT 15 57 SURP motif.
 DOMAIN 149 289 CID.
 DOMAIN 841 891 G-patch.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 18 18 N6-acetyllysine.
 MOD_RES 714 714 Phosphotyrosine (By similarity).
 MOD_RES 813 813 Phosphoserine.
 MOD_RES 815 815 Phosphoserine.
 MOD_RES 817 817 Phosphoserine.
 MOD_RES 819 819 Phosphothreonine.
 MOD_RES 828 828 Phosphoserine.
 MOD_RES 879 879 N6-acetyllysine.
 MOD_RES 904 904 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Endoplasmic reticulum; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 916 AA 
Protein Sequence
MEMPLPPDDQ ELRNVIDKLA QFVARNGPEF EKMTMEKQKD NPKFSFLFGG EFYSYYKCKL 60
ALEQQQLICK QQTPELEPAA TMPPLPQPPL APAAPIPPAQ GAPSMDELIQ QSQWNLQQQE 120
QHLLALRQEQ VTAAVAHAVE QQMQKLLEET QLDMNEFDNL LQPIIDTCTK DAISAGKNWM 180
FSNAKSPPHC ELMAGHLRNR ITADGAHFEL RLHLIYLIND VLHHCQRKQA RELLAALQKV 240
VVPIYCTSFL AVEEDKQQKI ARLLQLWEKN GYFDDSIIQQ LQSPALGLGQ YQATLINEYS 300
SVVQPVQLAF QQQIQTLKTQ HEEFVTSLAQ QQQQQQQQQQ QLQMPQMEAE VKATPPPPAP 360
PPAPAPAPAI PPTTQPDDSK PPIQMPGSSE YEAPGGVQDP AAAGPRGPGP HDQIPPNKPP 420
WFDQPHPVAP WGQQQPPEQP PYPHHQGGPP HCPPWNNSHE GMWGEQRGDP GWNGQRDAPW 480
NNQPDAAWNS QFEGPWNSQH EQPPWGGGQR EPPFRMQRPP HFRGPFPPHQ QHPQFNQPPH 540
PHNFNRFPPR FMQDDFPPRH PFERPPYPHR FDYPQGDFPA EMGPPHHHPG HRMPHPGINE 600
HPPWAGPQHP DFGPPPHGFN GQPPHMRRQG PPHINHDDPS LVPNVPYFDL PAGLMAPLVK 660
LEDHEYKPLD PKDIRLPPPM PPSERLLAAV EAFYSPPSHD RPRNSEGWEQ NGLYEFFRAK 720
MRARRRKGQE KRNSGPSRSR SRSKSRGRSS SRSNSRSSKS SGSYSRSRSR SCSRSYSRSR 780
SRSRSRSRSS RSRSRSQSRS RSKSYSPGRR RRSRSRSPTP PSSAGLGSNS APPIPDSRLG 840
EENKGHQMLV KMGWSGSGGL GAKEQGIQDP IKGGDVRDKW DQYKGVGVAL DDPYENYRRN 900
KSYSFIARMK ARDECK 916 
Gene Ontology
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0033017; C:sarcoplasmic reticulum membrane; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0008285; P:negative regulation of cell proliferation; IDA:MGI.
 GO:0007399; P:nervous system development; TAS:ProtInc.
 GO:0051533; P:positive regulation of NFAT protein import into nucleus; IMP:UniProtKB.
 GO:0051209; P:release of sequestered calcium ion into cytosol; IMP:UniProtKB.
 GO:0006396; P:RNA processing; IEA:InterPro. 
Interpro
 IPR006569; CID_dom.
 IPR008942; ENTH_VHS.
 IPR000467; G_patch_dom.
 IPR006903; RNA_pol_II-bd.
 IPR000061; Surp. 
Pfam
 PF04818; CTD_bind
 PF01585; G-patch
 PF01805; Surp 
SMART
 SM00443; G_patch
 SM00582; RPR
 SM00648; SWAP 
PROSITE
 PS51391; CID
 PS50174; G_PATCH
 PS50128; SURP 
PRINTS