CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022680
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein piccolo 
Protein Synonyms/Alias
 Aczonin; Brain-derived HLMN protein; Multidomain presynaptic cytomatrix protein 
Gene Name
 Pclo 
Gene Synonyms/Alias
 Acz 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1062GQLGDMDKMPPASSGubiquitination[1]
1326SDEKDLLKGLKKDSFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. 
Sequence Annotation
 DOMAIN 4424 4518 PDZ.
 DOMAIN 4637 4735 C2 1.
 DOMAIN 4952 5042 C2 2.
 ZN_FING 532 556 C4-type (Potential).
 ZN_FING 997 1020 C4-type (Potential).
 REGION 401 500 10 X 10 AA tandem approximate repeats of
 MOD_RES 625 625 Phosphoserine.
 MOD_RES 748 748 Phosphoserine.
 MOD_RES 860 860 Phosphothreonine.
 MOD_RES 1120 1120 Phosphothreonine.
 MOD_RES 1340 1340 Phosphoserine.
 MOD_RES 1341 1341 Phosphoserine.
 MOD_RES 1400 1400 Phosphoserine.
 MOD_RES 1439 1439 Phosphoserine.
 MOD_RES 1444 1444 Phosphoserine.
 MOD_RES 1457 1457 Phosphoserine.
 MOD_RES 1575 1575 Phosphoserine.
 MOD_RES 1585 1585 Phosphoserine.
 MOD_RES 1760 1760 Phosphothreonine.
 MOD_RES 1763 1763 Phosphoserine.
 MOD_RES 1766 1766 Phosphoserine.
 MOD_RES 1772 1772 Phosphoserine.
 MOD_RES 1808 1808 Phosphoserine.
 MOD_RES 1828 1828 Phosphotyrosine.
 MOD_RES 1829 1829 Phosphoserine.
 MOD_RES 2998 2998 Phosphothreonine.
 MOD_RES 3358 3358 Phosphoserine.
 MOD_RES 3376 3376 Phosphothreonine.
 MOD_RES 3513 3513 Phosphothreonine.
 MOD_RES 3545 3545 Phosphoserine.
 MOD_RES 3610 3610 Phosphoserine.
 MOD_RES 3616 3616 Phosphoserine.
 MOD_RES 3761 3761 Phosphotyrosine.
 MOD_RES 3763 3763 Phosphoserine.
 MOD_RES 3894 3894 Phosphothreonine.
 MOD_RES 3895 3895 Phosphothreonine.
 MOD_RES 3936 3936 Phosphoserine.
 MOD_RES 3952 3952 Phosphoserine.
 MOD_RES 4286 4286 Phosphoserine.
 MOD_RES 4290 4290 Phosphoserine.
 MOD_RES 4293 4293 Phosphoserine.
 MOD_RES 4325 4325 Phosphoserine.
 CARBOHYD 2686 2686 O-linked (GlcNAc).
 CARBOHYD 2960 2960 O-linked (GlcNAc).  
Keyword
 Alternative splicing; Calcium; Calcium/phospholipid-binding; Cell junction; Complete proteome; Glycoprotein; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Synapse; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 5068 AA 
Protein Sequence
MGNEASLEGE GLPEGLAAAA GGAGGSGSAL HPGIPAGMEA DLSQLSEEER RQIAAVMSRA 60
QGLPKGSVPA AAAESPSMHR KQELDSSQAP QQPGKPPDPG RPPQHGLSKS RTTDTFRSEQ 120
KLPGRSPSTI SLKESKSRTD FKEEYKSSMM PGFFSDVNPL SAVSSVVNKF NPFDLISDSE 180
AVQEETTKKQ KVAQKDQGKS EGITKPSLQQ PSPKLIPKQQ GPGKEVIPQD IPSKSVSSQQ 240
AEKTKPQAPG TAKPSQQSPA QTPAQQAKPV AQQPGPAKAT VQQPGPAKSP AQPAGTGKSP 300
AQPPVTAKPP AQQAGLEKTS LQQPGPKSLA QTPGQGKVPP GPAKSPAQQP GTAKLPAQQP 360
GPQTAAKVPG PTKTPAQLSG PGKTPAQQPG PTKPSPQQPI PAKPQPQQPV ATKPQPQQPA 420
PAKPQPQHPT PAKPQPQHPT PAKPQPQQPT PAKPQPQQPT PAKPQPQQPT PAKPQPQHPT 480
PAKPQPQQPG LGKPSAQQPS KSISQTVTGR PLQAPPTSAA QAPAQGLSKT ICPLCNTTEL 540
LLHTPEKANF NTCTECQSTV CSLCGFNPNP HLTEIKEWLC LNCQMQRALG GELAAIPSSP 600
QPTPKAASVQ PATASKSPVP SQQASPKKEL PSKQDSPKAP ESKKPPPLVK QPTLHGPTPA 660
TAPQPPVAEA LPKPAPPKKP SAALPEQAKA PVADVEPKQP KTTETLTDSP SSAAATSKPA 720
ILSSQVQAQA QVTTAPPLKT DSAKTSQSFP PTGDTITPLD SKAMPRPASD SKIVSHPGPT 780
SESKDPVQKK EEPKKAQTKV TPKPDTKPVP KGSPTPSGTR PTTGQATPQS QQPPKPPEQS 840
RRFSLNLGGI ADAPKSQPTT PQETVTGKLF GFGASIFSQA SNLISTAGQQ APHPQTGPAA 900
PSKQAPPPSQ TLAAQGPPKS TGPHPSAPAK TTAVKKETKG PAAENLEAKP VQAPTVKKAE 960
KDKKPPPGKV SKPPPTEPEK AVLAQKPDKT TKPKPACPLC RTELNVGSQD PPNFNTCTEC 1020
KNQVCNLCGF NPTPHLTEIQ EWLCLNCQTQ RAISGQLGDM DKMPPASSGP KASPVPAPAE 1080
PPPQKTPTAA HAKGKKKETE VKAETEKQIP EKETPSIEKT PPAVATDQKL EESEVTKSLV 1140
SVLPEKKPSE EEKALPADKK EKKPPAAEAP PLEEKKPIPD DQKLPPDAKP SASEGEEKRD 1200
LLKAHVQIPE EGPIGKVASL ACEGEQQPDT RPEDLPGATP QTLPKDRQKE SRDVTQPQAE 1260
GTAKEGRGEP SKDRTEKEED KSDTSSSQQP KSPQGLSDTG YSSDGISGSL GEIPSLIPSD 1320
EKDLLKGLKK DSFSQESSPS SPSDLAKLES TVLSILEAQA STLVGEKAEK KTQPQKVSPE 1380
QPQDQQKTQT PSETRDISIS EEEIKESQEK KVTSKKDSAQ GFPSRKEHKE NPELVDDLSP 1440
RRASYDSVED SSESENSPVA RRKRRTSIGS SSSEEYKQED SQGSGEDEDF IRKQIIEMSA 1500
DEDASGSEDE EFIRSQLKEI GGVTESQKRE ETKGKGKSPA GKHRRLTRKS STSFDDDAGR 1560
RHSWHDEDDE TFDESPELKF RETKSQESEE LVVAGGGGLR RFKTIELNST VTDKYSAESS 1620
QKKTTLYFDE EPELEMESLT DSPEDRSRGE GSSSLHASSF TPGTSPTSVS SLDEDSDSSP 1680
SHKKGESKQQ RKARHRSHGP LLPTIEDSSE EEELREEEEL LKEQEKQREL EQQQRKSSSK 1740
KSKKDKDELR AQRRRERPKT PPSNLSPIED ASPTEELRQA AEMEELHRSS CSEYSPSIES 1800
DPEGFEISPE KIIEVQKVYK LPTAVSLYSP TDEQSVMQKE GAQKALKSAE EMYEEMMHKP 1860
HKYKAFPAAN ERDEVFEKEP LYGGMLIEDY IYESLVEDTY NGSVDGSLLT RQDEQNGFMQ 1920
QRGREQKIRL QEQIYDDPMQ KITDLQKEFY ELESLHSIVP QEDIVSSSYI IPESHEIVDL 1980
GSMVTSTSEE KKLLDADAAY EELMKRQQMQ VTDGSSLIQT TMGDDMAEST LDFDRVQDAS 2040
LTSSILSGAS LTDSTSSATL SIPDVKITQH FSTEEFEDEY VTDYTREIQE IIAHESLILT 2100
YSEPSESATS VPPSDTPSLT SSISSVCTTD SSSPVTTLDS LTTVYTEPAD VITKFKDSEE 2160
ISSTYFPGSV IDYPEDIGVS LDRTITPESR TNADQIMISF PGIAPSITES VATKPERPQA 2220
DTISTDLPIS EKELIKGKKE TGDGIILEVL DAYKDKREES EAELTKISLP ETGLAPTPSS 2280
QTKEQPGSPH SVSGEILGQE KPTYRSPSGG LPVSTHPSKS HPFFRSSSLD ISAQPPPPPP 2340
PPPPPPPPPP PPPPPPLPPA TSPKPPTYPK RKLAAAAPVA PTAIVTAHAD AIPTVEATAA 2400
RRSNGLPATK ICAAAPPPVP PKPSSIPTGL VFTHRPEASK PPIAPKPAVP EIPVTTQKTT 2460
DTCPKPTGLP LTSNMSLNLV TSADYKLPSP TSPLSPHSNK SSPRYSKSLM ETYVVITLPS 2520
EPGTPTDSSA AQAITSWPLG SPPKDLVSLE TVFSVVPPMT STEIPSASQP TLYTSGALGT 2580
FSVTPAVTAS LFQTVPTSLT QFLPAEASKP EVSAVSSAVP SVAPRSVSIP IPPEPLALDR 2640
HQYKENGKLP LIGDAIDLRT IPKSEVKVTE KCMDLSASAM DVKRQTTANE VYRRQISAVQ 2700
PSIINLSAAS SLGTPVTMDS KTVAVVTCTD TTIYTTGTES QVGIEHAVTS PLQLTTSKHT 2760
ELQYRKPSSQ AFPMIRDEAP INLSLGPSTQ AVTLAVTKPV TVPPVGVTNG WTDSTISQGI 2820
TDGEVVDLST SKSHRTVVTM DESTSNVVTK IIEDEEKPVD LTAGRRAVCC DMVYKLPFGR 2880
SCTAQQPATT LPEDRFGYRD DHYQYDRSGP YGYRGIGGMK PSMSDTNLAE AGHFFYKSKN 2940
AFDYSGGTEA AVDLTSGRVS TGEVMDYSSK TTGPYPETRQ VISGVGISTP QYSTARMTPP 3000
PGPQYGVGSV LRSSNGVVYS SVATPIPSTF AITTQPGSIF STTVRDLSGI HTTDAITSLS 3060
ALHQSQPMPR SYFITTGASE TDISVTSIDI NASLQTITME TLPAETMDSV PTLTTASEVF 3120
SEVVGEESTL LIVPDEDKQQ QQLDLERELL ELEKIKQQRF AEELEWERQE IQRFREQEKI 3180
MVQKKLEELQ SMKQHLLYQQ EEERQAQFMM RQETLAQQQL QLEQIQQLQQ QLHQQLEEQK 3240
LRQIYQYNYE PSGTASPQTT TEQAILEGQY VATEGSQFWA TEDATTTAST VVAIEIPQSQ 3300
GWYTVQSDGV TQYIAPPGIL STVSEIPLTD VVVKEEKQPK KRSSGAKVRG QYDEMGESMA 3360
DDPRNLKKIV DSGVQTDDEE TADRTYASRR RRTKKSVDTS VQTDDEDQDE WDMPSRSRRK 3420
ARTGKYGDST AEGDKTKPPS KVSSVAVQTV AEISVQTEPL GTIRTPSIRA RVDAKVEIIK 3480
HISAPEKTYK GGSLGCQTET DPDTQSPPYM GATSPPKDKK RPTPLEIGYS SSHLRADPTV 3540
QLAPSPPKSP KVLYSPISPL SPGHALEPAF VPYEKPLPDD ISPQKVLHPD MAKVPPASPK 3600
TAKMMQRSMS DPKPLSPTAD ESSRAPFQYS EGFTAKGSQT TSGTQKKVKR TLPNPPPEEA 3660
STGTQSTYST MGTASRRRMC RTNTMARAKI LQDIDRELDL VERESAKLRK KQAELDEEEK 3720
EIDAKLRYLE MGINRRKEAL LKEREKRERA YLQGVAEDRD YMSDSEVSST RPSRVESQHG 3780
IERPRTAPQT EFSQFIPPQT QTEAQLVPPT SPYTQYQYSS PALPTQAPTP YTQQSHFQQQ 3840
TLYHQQVSPY QTQPTFQAVA TMSFTPQAQP TPTPQPSYQL PSQMMVIQQK PRQTTLYLEP 3900
KITSTYEVIR NQPLMIAPVS TDNTYAVSHL GSKYNSLDLR IGLEERSSMA SSPISSISAD 3960
SFYADIDHHT SRNYVLIDDI GDITKGTAAL SSAFSLHEKD LSKTDRLLRT TETRRSQEVT 4020
DFLAPLQTSS RLHSYVKAEE DSMEDPYELK LLKHQIKQEF RRGTESLDHL AGLSHYYHAD 4080
TSYRHFPKSE KYSISRLTLE KQAAKQLPAA ILYQKQSKHK KALIDPKMSK FSPIQESRDL 4140
EPDYPTYLSS STSSIGGISS RARLLQDDIT FGLRKNITDQ QKFMGSSLGS GLGTLGNTIR 4200
SALQDEADKP YSSGSRSRPS SRPSSVYGLD LSIKRDSSSS SLRLKAQEAE ALDVSFGHSS 4260
SSARTKPTSL PISQSRGRIP IVAQNSEEES PLSPVGQPMG MARAAAGPLP PISADTRDQF 4320
GSSHSLPEVQ QHMREESRTR GYDRDIAFIM DDFQHAMSDS EAYHLRREET DWFDKPRESR 4380
LENGHGLDRK LPERLVHSRP LSQHQEQILQ MNGKTMHYIF PHARIKITRD SKDHTVSGNG 4440
LGIRIVGGKE IPGHSGEIGA YIAKILPGGS AEHSGKLIEG MQVLEWNGIP LTSKTYEEVQ 4500
SIINQQSGEA EICVRLDLNM LSDSENPQHL ELHEPPKVVD KAKSPGVDPK QLAAELQKVS 4560
LQQSPLVMSS VVEKGAHAHS GPTSAGSSSV PSPGQPGSPS VSKKKHGGSK PTDVSKTASH 4620
PITGEIQLQI NYDLGNLIIH ILQARNLVPR DNNGYSDPFV KVYLLPGRGQ VMVVQNASVE 4680
YKRRTKYVQK SLNPEWNQTV IYKSISMEQL MKKTLEVTVW DYDRFSSNDF LGEVLIDLSS 4740
TSHLDNTPRW YPLKEQTESI EHGKSHSSQN SQQSPKPSVI KSRSHGIFPD PSKDMQVPTI 4800
EKSHSSPGSS KSSSEGHLRS HGPSRSQSKT SVAQTHLEDA GAAIAAAEAA VQQLRIQPTK 4860
PTNHRPAETS VSTGSSGSSV GSGYSVDSEG SSCVAGEPNL LPIPRIGKMG QNGQDPVKQP 4920
GMGAADTEAK TQVMGEIKLA LKKEMKTDGE QLIVEILQCR NITYKFKSPD HLPDLYVKIY 4980
VINIATQKKV IKKKTRVCRH DREPSFNETF RFSLSPAGHS LQILLFSNGG KFMKKTLIGE 5040
ACIWLDKVDL RKRIVNWHKL LMSPTQTH 5068 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0045202; C:synapse; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; ISS:UniProtKB.
 GO:0005544; F:calcium-dependent phospholipid binding; ISS:UniProtKB.
 GO:0005522; F:profilin binding; IDA:UniProtKB.
 GO:0019933; P:cAMP-mediated signaling; IDA:MGI.
 GO:0007010; P:cytoskeleton organization; IDA:MGI.
 GO:0030073; P:insulin secretion; IDA:MGI.
 GO:0017157; P:regulation of exocytosis; IDA:MGI.
 GO:0016080; P:synaptic vesicle targeting; NAS:UniProtKB. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR001478; PDZ.
 IPR011011; Znf_FYVE_PHD.
 IPR008899; Znf_piccolo.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00168; C2
 PF00595; PDZ
 PF05715; zf-piccolo 
SMART
 SM00239; C2
 SM00228; PDZ 
PROSITE
 PS50004; C2
 PS50106; PDZ 
PRINTS