CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005816
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mannose-6-phosphate isomerase 
Protein Synonyms/Alias
 Phosphohexomutase; Phosphomannose isomerase; PMI 
Gene Name
 PMI40 
Gene Synonyms/Alias
 YER003C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
73SLRDIISKNPSAMLGacetylation[1]
81NPSAMLGKDIIDKFHacetylation[1]
114SIQAHPDKALGKILHacetylation[1]
214VMNASDDKIKIQARSubiquitination[2]
326YTYDPVEKQKMQPLKacetylation[1]
333KQKMQPLKFDRSSGNacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. 
Sequence Annotation
 ACT_SITE 300 300 By similarity.
 METAL 109 109 Zinc (By similarity).
 METAL 111 111 Zinc (By similarity).
 METAL 136 136 Zinc (By similarity).
 METAL 281 281 Zinc (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 107 107 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Isomerase; Metal-binding; Phosphoprotein; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 429 AA 
Protein Sequence
MSNKLFRLDA GYQQYDWGKI GSSSAVAQFA AHSDPSVQIE QDKPYAELWM GTHSKMPSYN 60
HESKESLRDI ISKNPSAMLG KDIIDKFHAT NELPFLFKVL SIEKVLSIQA HPDKALGKIL 120
HAQDPKNYPD DNHKPEMAIA VTDFEGFCGF KPLQEIADEL KRIPELRNIV GEETSRNFIE 180
NIQPSAQKGS PEDEQNKKLL QAVFSRVMNA SDDKIKIQAR SLVERSKNSP SDFNKPDLPE 240
LIQRLNKQFP DDVGLFCGCL LLNHCRLNAG EAIFLRAKDP HAYISGDIME CMAASDNVVR 300
AGFTPKFKDV KNLVSMLTYT YDPVEKQKMQ PLKFDRSSGN GKSVLYNPPI EEFAVLETTF 360
DEKLGQRHFE GVDGPSILIT TKGNGYIKAD GQKLKAEPGF VFFIAPHLPV DLEAEDEAFT 420
TYRAFVEPN 429 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004476; F:mannose-6-phosphate isomerase activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000032; P:cell wall mannoprotein biosynthetic process; IMP:SGD.
 GO:0009298; P:GDP-mannose biosynthetic process; IMP:SGD.
 GO:0006486; P:protein glycosylation; IMP:SGD. 
Interpro
 IPR001250; Man6P_Isoase-1.
 IPR016305; Mannose-6-P_Isomerase.
 IPR018050; Pmannose_isomerase-type1_CS.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin. 
Pfam
 PF01238; PMI_typeI 
SMART
  
PROSITE
 PS00965; PMI_I_1
 PS00966; PMI_I_2 
PRINTS
 PR00714; MAN6PISMRASE.