CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024161
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin-like protein 6A 
Protein Synonyms/Alias
 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; BRG1-associated factor 53A; BAF53A 
Gene Name
 Actl6a 
Gene Synonyms/Alias
 Actl6; Baf53a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
303DFGAERLKIPEGLFDubiquitination[1]
379TPPSMRLKLIANNTTacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 233 233 Phosphoserine (By similarity).  
Keyword
 Acetylation; Activator; Chromatin regulator; Complete proteome; DNA damage; DNA recombination; DNA repair; Growth regulation; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 429 AA 
Protein Sequence
MSGGVYGGDE VGALVFDIGS YTVRAGYAGE DCPKVDFPTA IGVVLERDDG STMMEIDGDK 60
GKQGGPTYYI DTNALRVPRE NMEAISPLKN GMVEDWDSFQ AILDHTYKMH VKSEASLHPV 120
LMSEAPWNTR AKREKLTELM FEHYSIPAFF LCKTAVLTAF ANGRSTGLIL DSGATHTTAI 180
PVHDGYVLQQ GIVKSPLAGD FITMQCRELF QEMNIELIPP YMIASKEAVR EGSPANWKRK 240
EKLPQVTRSW HNYMCNCVIQ DFQASVLQVS DSTYDEQVAA QMPTVHYEFP NGYNCDFGAE 300
RLKIPEGLFD PSNVKGLSGN TMLGVSHVVT TSVGMCDIDI RPGLYGSVIV AGGNTLIQSF 360
TDRLNRELSQ KTPPSMRLKL IANNTTVERR FSSWIGGSIL ASLGTFQQMW ISKQEYEEGG 420
KQCVERKCP 429 
Gene Ontology
 GO:0031011; C:Ino80 complex; IEA:Compara.
 GO:0071564; C:npBAF complex; IDA:UniProtKB.
 GO:0035267; C:NuA4 histone acetyltransferase complex; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0016514; C:SWI/SNF complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0006338; P:chromatin remodeling; IDA:MGI.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0043968; P:histone H2A acetylation; ISS:UniProtKB.
 GO:0043967; P:histone H4 acetylation; ISS:UniProtKB.
 GO:0007399; P:nervous system development; IMP:UniProtKB.
 GO:0003407; P:neural retina development; IEA:Compara.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR004000; Actin-related.
 IPR004001; Actin_CS. 
Pfam
 PF00022; Actin 
SMART
 SM00268; ACTIN 
PROSITE
 PS00406; ACTINS_1
 PS00432; ACTINS_2 
PRINTS
 PR00190; ACTIN.