CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020091
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Apoptosis-inducing factor 2 
Protein Synonyms/Alias
 Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; p53-responsive gene 3 protein 
Gene Name
 AIFM2 
Gene Synonyms/Alias
 AMID; PRG3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63SVETGFAKKTFISYSubiquitination[1]
193PSVRQEVKEILLRKGubiquitination[1, 2, 3, 4]
225NEYREYIKVQTDKGTubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest. 
Sequence Annotation
 NP_BIND 18 22 FAD (Potential).
 BINDING 54 54 FAD (Potential).
 BINDING 82 82 FAD; via amide nitrogen and carbonyl
 BINDING 285 285 FAD (Potential).
 LIPID 2 2 N-myristoyl glycine.  
Keyword
 Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; DNA-binding; FAD; Flavoprotein; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; Oxidoreductase; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 373 AA 
Protein Sequence
MGSQVSVESG ALHVVIVGGG FGGIAAASQL QALNVPFMLV DMKDSFHHNV AALRASVETG 60
FAKKTFISYS VTFKDNFRQG LVVGIDLKNQ MVLLQGGEAL PFSHLILATG STGPFPGKFN 120
EVSSQQAAIQ AYEDMVRQVQ RSRFIVVVGG GSAGVEMAAE IKTEYPEKEV TLIHSQVALA 180
DKELLPSVRQ EVKEILLRKG VQLLLSERVS NLEELPLNEY REYIKVQTDK GTEVATNLVI 240
LCTGIKINSS AYRKAFESRL ASSGALRVNE HLQVEGHSNV YAIGDCADVR TPKMAYLAGL 300
HANIAVANIV NSVKQRPLQA YKPGALTFLL SMGRNDGVGQ ISGFYVGRLM VRLTKSRDLF 360
VSTSWKTMRQ SPP 373 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005811; C:lipid particle; IDA:UniProtKB.
 GO:0005741; C:mitochondrial outer membrane; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0004174; F:electron-transferring-flavoprotein dehydrogenase activity; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:UniProtKB.
 GO:0008637; P:apoptotic mitochondrial changes; NAS:UniProtKB.
 GO:0030261; P:chromosome condensation; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IMP:UniProtKB. 
Interpro
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom.
 IPR000103; Pyridine_nuc-diS_OxRdtase_2. 
Pfam
 PF00070; Pyr_redox
 PF07992; Pyr_redox_2 
SMART
  
PROSITE
  
PRINTS
 PR00368; FADPNR.
 PR00469; PNDRDTASEII.