UniProt Accession

 GUTQ_ECOLI; P17115; Q2MAC1; Q46874 

Genbank Protein ID

 U29579; U00096; AP009048; X51361 

Genbank Nucleotide ID

 AAA69217.1; AAC75750.2; BAE76785.1; CAA35745.1 

Protein Name

 Arabinose 5-phosphate isomerase GutQ 

Protein Synonyms/Alias

 API; G-API; Phosphosugar aldol-ketol isomerase 

Gene Name

 gutQ 

Gene Synonyms/Alias

 srlQ; b2708; JW5431 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
52	VVVSGIGKSGHIGKK	acetylation	[1]
58	GKSGHIGKKIAATLA	acetylation	[1]
115	IIPRLEDKSIALLAM	acetylation	[1]
125	ALLAMTGKPTSPLGL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P). It appears that the physiological function of G-API may be to synthesize the regulatory molecule A5P, which in turn participates in the induction of the gut operon through an unknown mechanism. It is also able of sustaining the biosynthetic pathway of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs). 

Sequence Annotation

 DOMAIN 34 177 SIS.
 DOMAIN 203 261 CBS 1.
 DOMAIN 269 321 CBS 2.
 NP_BIND 49 54 ATP (Potential).
 REGION 68 69 Substrate binding (By similarity).
 REGION 107 116 Substrate binding (By similarity).
 REGION 141 143 Substrate binding (By similarity).
 METAL 75 75 Zinc (By similarity).
 BINDING 75 75 Substrate (By similarity).
 BINDING 81 81 Substrate (By similarity).
 BINDING 215 215 Substrate (By similarity).
 BINDING 267 267 Substrate (By similarity).  

Keyword

 ATP-binding; CBS domain; Complete proteome; Direct protein sequencing; Isomerase; Lipopolysaccharide biosynthesis; Metal-binding; Nucleotide-binding; Reference proteome; Repeat; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 321 AA 

Protein Sequence

Gene Ontology

 GO:0019146; F:arabinose-5-phosphate isomerase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042710; P:biofilm formation; IMP:EcoCyc.
 GO:0019294; P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IMP:UniProtKB. 

Interpro

 IPR000644; Cysta_beta_synth_core.
 IPR004800; KdsD/KpsF-type.
 IPR001347; SIS. 

Pfam

 PF00571; CBS
 PF01380; SIS 

SMART

 SM00116; CBS 

PROSITE

 PS51371; CBS
 PS51464; SIS 

PRINTS