CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036381
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Heat shock 70 kDa protein 4L 
Protein Synonyms/Alias
  
Gene Name
 HSPA4L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53RAIGNAAKSQIVTNVubiquitination[1, 2, 3, 4, 5]
93RLPYELQKMPNGSAGubiquitination[3]
153DLPPLDEKPRNVVFIubiquitination[6]
208DYFCDEFKTKYKINVubiquitination[1, 2]
310ATRIPAVKEQITKFFubiquitination[3, 5]
315AVKEQITKFFLKDISubiquitination[3, 7]
319QITKFFLKDISTTLNubiquitination[1, 2, 4]
366YSITLRWKTSFEDGSubiquitination[6]
381GECEVFCKNHPAPFSubiquitination[2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MSVVGIDLGF LNCYIAVARS GGIETIANEY SDRCTPACIS LGSRTRAIGN AAKSQIVTNV 60
RNTIHGFKKL HGRSFDDPIV QTERIRLPYE LQKMPNGSAG VKIPSFFTDA ERRSVMAAAQ 120
VAGLNCLRLM NETTAVALAY GIYKQDLPPL DEKPRNVVFI DMGHSAYQVL VCAFNKGKLK 180
VLATTFDPYL GGRNFDEALV DYFCDEFKTK YKINVKENSR ALLRLYQECE KLKKLMSANA 240
SDLPLNIECF MNDLDVSSKM NRAQFEQLCA SLLARVEPPL KAVMEQANLQ REDISSIEIV 300
GGATRIPAVK EQITKFFLKD ISTTLNADEA VARGCALQCA ILSPAFKVRE FSITDLVPYS 360
ITLRWKTSFE DGSGECEVFC KNHPAPFSKV ITFHKKEPFE LEAFYTNLHE VPYPDARIGS 420
FTIQNVFPQS DGDSSKVKVK VRVNIHGIFS VASASVIEKQ NLEGDHSDAP METETSFKNE 480
NKDNMDKMQV DQEEGHQKCH AEHTPEEEID HTGAKTKSAV SDKQDRLNQT L 531 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW. 
Interpro
 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.