UniProt Accession

 RFA2_HUMAN; P15927; Q52II0; Q5TEI9; Q5TEJ5 

Genbank Protein ID

 J05249; CR450348; DQ001128; AL109927; AL109927; AL109927; BC001630; BC012157; BC021257 

Genbank Nucleotide ID

 AAA36560.1; CAG29344.1; AAX84514.1; CAI21777.1; CAI21778.1; CAI21775.1; AAH01630.1; AAH12157.1; AAH21257.1 

Protein Name

 Replication protein A 32 kDa subunit 

Protein Synonyms/Alias

 RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34 

Gene Name

 RPA2 

Gene Synonyms/Alias

 REPA2; RPA32; RPA34 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
101	APTNIVYKIDDMTAA	ubiquitination	[1]
135	VPPETYVKVAGHLRS	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. 

Sequence Annotation

 REGION 187 270 Interaction with TIPIN (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 21 21 Phosphothreonine; by PRKDC; in vitro.
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 29 29 Phosphoserine; by CDK1; in vitro.
 MOD_RES 33 33 Phosphoserine; by PRKDC; in vitro.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 270 AA 

Protein Sequence

Gene Ontology

 GO:0005662; C:DNA replication factor A complex; IPI:MGI.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003697; F:single-stranded DNA binding; TAS:ProtInc.
 GO:0000730; P:DNA recombinase assembly; TAS:Reactome.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0006297; P:nucleotide-excision repair, DNA gap filling; TAS:Reactome.
 GO:0010569; P:regulation of double-strand break repair via homologous recombination; IMP:UniProtKB.
 GO:0000722; P:telomere maintenance via recombination; TAS:Reactome.
 GO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase.
 IPR014646; RPA32.
 IPR014892; RPA_C.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF08784; RPA_C
 PF01336; tRNA_anti 

SMART

  

PROSITE

  

PRINTS