CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036816
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Uncharacterized protein 
Protein Synonyms/Alias
  
Gene Name
 EPN2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11SSIRRQMKNIVNNYSubiquitination[1]
107IFAIQTLKDFQYIDRubiquitination[1, 2, 3, 4]
117QYIDRDGKDQGINVRubiquitination[2, 4]
135KQLVALLKDEERLKAubiquitination[2, 4, 5]
188HSEQEYGKAGGSPASubiquitination[2, 3, 4, 5]
366QPASSAGKRASDAWGubiquitination[2, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 499 AA 
Protein Sequence
MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV VAFSEIMSMV 60
WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI FAIQTLKDFQ YIDRDGKDQG 120
INVREKSKQL VALLKDEERL KAERAQALKT KERMAQVATG MGSNQITFGR GSSQPNLSTS 180
HSEQEYGKAG GSPASYHGST SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQEERL 240
RRGDDLRLQM ALEESRRDTV KIPKKKEHGS LPQQTTLLDL MDALPSSGPA AQKAEPWGPS 300
ASTNQTNPWG GPAAPASTSD PWPSFGTKPA ASIDPWGVPT GATVQSVPKN SDPWAASQQP 360
ASSAGKRASD AWGAVSTTKP VSVSGSFELF SNLNGTIKDD FSEFDNLRTS KKTAESVTSL 420
PSQNNGTTSP DPFESQPLTV ASSKPSSARK TPESFLGPNA ALVNLDSLVT RPAPPAQSLN 480
PFLAPGMQGL TGLLFTMEI 499 
Gene Ontology
 GO:0030128; C:clathrin coat of endocytic vesicle; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:InterPro. 
Interpro
 IPR008942; ENTH_VHS.
 IPR027319; Epsin-2_metazoa.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF01417; ENTH 
SMART
 SM00273; ENTH
 SM00726; UIM 
PROSITE
 PS50942; ENTH
 PS50330; UIM 
PRINTS