| Tag | Content |
|---|
| CPLM ID | CPLM-016146 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Protein argonaute 9 |
Protein Synonyms/Alias | |
Gene Name | AGO9 |
Gene Synonyms/Alias | At5g21150; T10F18.180 |
Created Date | July 27, 2013 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
NCBI Taxa ID | 3702 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 464 | NLSPIKGKWNFMRKT | ubiquitination | [1] |
|
Reference | [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants. Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K. Mol Cell Proteomics. 2007 Apr;6(4):601-10. [ PMID: 17272265] |
Functional Description | Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates preferentially with small RNAs of 24 nucleotide in length with a 5' terminal adenosine. Interacts with 24 nucleotide sRNAs derived from transposable elements (TEs). Required to silence pericentrometric-located TEs in female gametes and their accessory cells. Necessary to inactivate a significant proportion of long terminal repeat retrotransposons (LTRs) in the ovule. Required to specify cell fate in ovule. Involved in the control of female gamete formation by restricting the specification of gametophyte precursors in a dosage-dependent, non-cell-autonomous manner. Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO9 function in RNA silencing. |
Sequence Annotation | DOMAIN 264 380 PAZ.
DOMAIN 550 857 Piwi.
CROSSLNK 464 464 Glycyl lysine isopeptide (Lys-Gly) |
Keyword | Complete proteome; Isopeptide bond; Plant defense; Reference proteome; Repressor; Ribonucleoprotein; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation; Ubl conjugation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 896 AA |
Protein Sequence | MDSDEPNGSG LPPPPPFVPA NLVPEVEPVK KNILLPMARP RGSGSKGQKI PLLTNHFGVK 60
FNKPSGYFFH YSVAINYEDG RPVEAKGIGR KILDKVQETY QSDLGAKYFA YDGEKTLFTV 120
GALPSNKLDF SVVLEEIPSS RNHAGNDTND ADRKRSRRPN QTKKFMVEIS YAAKIPMQAI 180
ASALQGKETE NLQDALRVLD IILRQSAARQ GCLLVRQSFF HNDVKNFVPI GGGVSGCRGF 240
HSSFRTTQGG LSLNIDTSTT MIVQPGPVVD FLLANQNKKD PYGMDWNKAR RVLKNLRVQI 300
TLSNREYKIS GLSEHSCKDQ LFTWRKPNDK GEFEEVEITV LNYYKERNIE VRYSGDFPCI 360
NVGKPKRPTY FPIEFCNLVS LQRYTKSLTN FQRAALVEKS RQKPPERMAS LTKGLKDSNY 420
NADPVLQDSG VSIITNFTQV EGRILPTPML KVGKGENLSP IKGKWNFMRK TLAEPTTVTR 480
WAVVNFSARC DTNTLIRDLI KCGREKGINV EPPFKDVINE NPQFRNAPAT VRVENMFEQI 540
KSKLPKPPLF LLCILAERKN SDVYGPWKKK NLVDLGIVTQ CIAPTRLNDQ YLTNVLLKIN 600
AKLGGLNSLL AMERSPAMPK VTQVPTIIVG MDVSHGSPGQ SDIPSIAAVV SSRQWPLISK 660
YKACVRTQSR KMEMIDNLFK PVNGKDEGMF RELLLDFYYS SENRKPEHII IFRDGVSESQ 720
FNQVLNIELD QMMQACKFLD DTWHPKFTVI VAQKNHHTKF FQSRGPDNVP PGTIIDSQIC 780
HPRNFDFYLC AHAGMIGTTR PTHYHVLYDE IGFATDDLQE LVHSLSYVYQ RSTTAISVVA 840
PVCYAHLAAA QMGTVMKYEE LSETSSSHGG ITTPGAVPVP PMPQLHNNVS TSMFFC 896 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:TAIR. GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW. GO:0035197; F:siRNA binding; IDA:TAIR. GO:0006952; P:defense response; IEA:UniProtKB-KW. GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW. GO:0010529; P:negative regulation of transposition; IMP:TAIR. GO:0048481; P:ovule development; IMP:TAIR. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |