CPLM-010397

Genbank Nucleotide ID

Nucleolar protein 14

Protein Synonyms/Alias

Nucleolar complex protein 14

C4orf9; NOL14; RES4-25

Homo sapiens (Human)

Position	Peptide	Type	References
26	GARGGPAKANSNPFE	ubiquitination	[1]
35	NSNPFEVKVNRQKFQ	ubiquitination	[1]
226	EKLDQDWKEIQTLLS	ubiquitination	[1]
249	RDKKEKPKPDAYDMM	ubiquitination	[1]
265	RELGFEMKAQPSNRM	ubiquitination	[2]
411	GKGLISGKERAGKAT	acetylation	[3]
535	MEEMIETKGRAALPG	ubiquitination	[1]
643	HPFRALGKNSELLVV	ubiquitination	[2]
772	LFTPRLVKVLEFGRK	acetylation	[4]
772	LFTPRLVKVLEFGRK	ubiquitination	[2, 5]
834	AERKRKVKQLFNSLA	ubiquitination	[2, 6, 7]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm (By similarity).

MOD_RES 96 96 Phosphoserine.
MOD_RES 146 146 Phosphoserine.
MOD_RES 148 148 Phosphoserine.
MOD_RES 349 349 Phosphoserine.

Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ribosome biogenesis; rRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; ISS:UniProtKB.
GO:0030692; C:Noc4p-Nop14p complex; ISS:UniProtKB.
GO:0005730; C:nucleolus; ISS:UniProtKB.
GO:0032040; C:small-subunit processome; ISS:UniProtKB.
GO:0030515; F:snoRNA binding; ISS:UniProtKB.
GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ISS:UniProtKB.
GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ISS:UniProtKB.
GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ISS:UniProtKB.