CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002989
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent protease subunit HslV 
Protein Synonyms/Alias
 Heat shock protein HslV 
Gene Name
 hslV 
Gene Synonyms/Alias
 htpO; yiiC; b3932; JW3903 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
64LFELFERKLEMHQGHacetylation[1]
74MHQGHLVKAAVELAKacetylation[1, 2]
81KAAVELAKDWRTDRMacetylation[1, 2]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111
Functional Description
 Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. The complex has been shown to be involved in the specific degradation of heat shock induced transcription factors such as RpoH and SulA. In addition, small hydrophobic peptides are also hydrolyzed by HslV. HslV has weak protease activity even in the absence of HslU, but this activity is induced more than 100-fold in the presence of HslU. HslU recognizes protein substrates and unfolds these before guiding them to HslV for hydrolysis. HslV is not believed to degrade folded proteins. 
Sequence Annotation
 ACT_SITE 2 2
 METAL 157 157 Sodium; via carbonyl oxygen.
 METAL 160 160 Sodium; via carbonyl oxygen.
 METAL 163 163 Sodium; via carbonyl oxygen.  
Keyword
 3D-structure; Allosteric enzyme; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Protease; Reference proteome; Sodium; Stress response; Threonine protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 176 AA 
Protein Sequence
MTTIVSVRRN GHVVIAGDGQ ATLGNTVMKG NVKKVRRLYN DKVIAGFAGG TADAFTLFEL 60
FERKLEMHQG HLVKAAVELA KDWRTDRMLR KLEALLAVAD ETASLIITGN GDVVQPENDL 120
IAIGSGGPYA QAAARALLEN TELSAREIAE KALDIAGDIC IYTNHFHTIE ELSYKA 176 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0009376; C:HslUV protease complex; IEA:HAMAP.
 GO:0005839; C:proteasome core complex; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0051603; P:proteolysis involved in cellular protein catabolic process; IEA:InterPro.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 
Interpro
 IPR022281; ATP-dep_Prtase_HsIV_su.
 IPR001353; Proteasome_sua/b. 
Pfam
 PF00227; Proteasome 
SMART
  
PROSITE
  
PRINTS