UniProt Accession

 BNIP3_HUMAN; Q12983; O14620; Q96GP0 

Genbank Protein ID

 U15174; AF002697; AY886764; AL162274; BC009342; BC021989 

Genbank Nucleotide ID

 AAC00022.1; AAC16738.1; AAW62256.1; CAD13201.1; AAH21989.1 

Protein Name

 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 

Protein Synonyms/Alias

  

Gene Name

 BNIP3 

Gene Synonyms/Alias

 NIP3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
90	DTHSIGEKNSSQSEE	ubiquitination	[1]
104	EDDIERRKEVESILK	ubiquitination	[1]
111	KEVESILKKNSDWIW	ubiquitination	[2]
135	PPKEFLFKHPKRTAT	ubiquitination	[2]
152	MRNTSVMKKGGIFSA	ubiquitination	[1]
153	RNTSVMKKGGIFSAE	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. 

Sequence Annotation

 MOTIF 100 125 BH3.
 MOD_RES 86 86 Phosphoserine.
 MOD_RES 92 92 Phosphoserine (By similarity).
 MOD_RES 95 95 Phosphoserine (By similarity).  

Keyword

 3D-structure; Apoptosis; Complete proteome; Host-virus interaction; Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 194 AA 

Protein Sequence

Gene Ontology

 GO:0030425; C:dendrite; ISS:UniProtKB.
 GO:0031307; C:integral to mitochondrial outer membrane; IDA:UniProtKB.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; ISS:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0006309; P:apoptotic DNA fragmentation; IDA:UniProtKB.
 GO:0048102; P:autophagic cell death; IEA:Compara.
 GO:0050873; P:brown fat cell differentiation; IEA:Compara.
 GO:0071279; P:cellular response to cobalt ion; IMP:BHF-UCL.
 GO:0070301; P:cellular response to hydrogen peroxide; IEA:Compara.
 GO:0071456; P:cellular response to hypoxia; IMP:BHF-UCL.
 GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB.
 GO:0006338; P:chromatin remodeling; IDA:UniProtKB.
 GO:0051607; P:defense response to virus; IDA:UniProtKB.
 GO:0008626; P:granzyme-mediated apoptotic signaling pathway; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IDA:UniProtKB.
 GO:0097193; P:intrinsic apoptotic signaling pathway; IEA:Compara.
 GO:0043653; P:mitochondrial fragmentation involved in apoptotic process; IDA:BHF-UCL.
 GO:0097345; P:mitochondrial outer membrane permeabilization; IDA:UniProtKB.
 GO:0035694; P:mitochondrial protein catabolic process; IMP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
 GO:0045837; P:negative regulation of membrane potential; IDA:UniProtKB.
 GO:0010637; P:negative regulation of mitochondrial fusion; IDA:BHF-UCL.
 GO:0051402; P:neuron apoptotic process; ISS:UniProtKB.
 GO:0090141; P:positive regulation of mitochondrial fission; IDA:BHF-UCL.
 GO:0043243; P:positive regulation of protein complex disassembly; IDA:BHF-UCL.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IDA:BHF-UCL.
 GO:0072593; P:reactive oxygen species metabolic process; IDA:UniProtKB.
 GO:0055093; P:response to hyperoxia; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR010548; BNIP3. 

Pfam

 PF06553; BNIP3 

SMART

  

PROSITE

 PS01259; BH3 

PRINTS