CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005514
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase PP-Z1 
Protein Synonyms/Alias
  
Gene Name
 PPZ1 
Gene Synonyms/Alias
 YML016C; YM9571.02C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
146EPKSPILKTNNSSTHubiquitination[1]
178TDDDNDDKDNDISHTubiquitination[1]
248ASSLHSRKSSFGSDGubiquitination[1]
378LDAGYAAKRTKNVCLubiquitination[1]
468LLFCYKIKYPENFFLacetylation[2]
468LLFCYKIKYPENFFLubiquitination[1]
593AINKFLNKFGFDLVCubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Essential for the maintenance of cell size and integrity in response to osmotic stress. 
Sequence Annotation
 ACT_SITE 480 480 Proton donor (By similarity).
 METAL 419 419 Iron (By similarity).
 METAL 421 421 Iron (By similarity).
 METAL 447 447 Iron (By similarity).
 METAL 447 447 Manganese (By similarity).
 METAL 479 479 Manganese (By similarity).
 METAL 528 528 Manganese (By similarity).
 METAL 603 603 Manganese (By similarity).
 MOD_RES 49 49 Phosphoserine.
 MOD_RES 171 171 Phosphothreonine.
 MOD_RES 222 222 Phosphoserine.
 MOD_RES 261 261 Phosphothreonine.
 MOD_RES 265 265 Phosphoserine.
 MOD_RES 690 690 Phosphoserine.
 LIPID 2 2 N-myristoyl glycine (Potential).  
Keyword
 Complete proteome; Hydrolase; Iron; Lipoprotein; Manganese; Metal-binding; Myristate; Phosphoprotein; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 692 AA 
Protein Sequence
MGNSSSKSSK KDSHSNSSSR NPRPQVSRTE TSHSVKSAKS NKSSRSRRSL PSSSTTNTNS 60
NVPDPSTPSK PNLEVNHQRH SSHTNRYHFP SSSHSHSNSQ NELLTTPSSS STKRPSTSRR 120
SSYNTKAAAD LPPSMIQMEP KSPILKTNNS STHVSKHKSS YSSTYYENAL TDDDNDDKDN 180
DISHTKRFSR SSNSRPSSIR SGSVSRRKSD VTHEEPNNGS YSSNNQENYL VQALTRSNSH 240
ASSLHSRKSS FGSDGNTAYS TPLNSPGLSK LTDHSGEYFT SNSTSSLNHH SSRDIYPSKH 300
ISNDDDIENS SQLSNIHASM ENVNDKNNNI TDSKKDPNEE FNDIMQSSGN KNAPKKFKKP 360
IDIDETIQKL LDAGYAAKRT KNVCLKNNEI LQICIKAREI FLSQPSLLEL SPPVKIVGDV 420
HGQYGDLLRL FTKCGFPPSS NYLFLGDYVD RGKQSLETIL LLFCYKIKYP ENFFLLRGNH 480
ECANVTRVYG FYDECKRRCN IKIWKTFIDT FNTLPLAAIV AGKIFCVHGG LSPVLNSMDE 540
IRHVVRPTDV PDFGLINDLL WSDPTDSPNE WEDNERGVSY CYNKVAINKF LNKFGFDLVC 600
RAHMVVEDGY EFFNDRSLVT VFSAPNYCGE FDNWGAVMSV SEGLLCSFEL LDPLDSAALK 660
QVMKKGRQER KLANQQQQMM ETSITNDNES QQ 692 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0019897; C:extrinsic to plasma membrane; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0048037; F:cofactor binding; IEA:InterPro.
 GO:0004724; F:magnesium-dependent protein serine/threonine phosphatase activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:SGD.
 GO:0034613; P:cellular protein localization; IMP:SGD.
 GO:0006883; P:cellular sodium ion homeostasis; IGI:SGD.
 GO:0006470; P:protein dephosphorylation; IMP:SGD. 
Interpro
 IPR004843; Metallo_PEstase_dom.
 IPR011159; PPPtase_PPZ.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 
Pfam
 PF00149; Metallophos 
SMART
 SM00156; PP2Ac 
PROSITE
 PS00125; SER_THR_PHOSPHATASE 
PRINTS
 PR00114; STPHPHTASE.