CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022136
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositide phosphatase SAC1 
Protein Synonyms/Alias
 Suppressor of actin mutations 1-like protein 
Gene Name
 SACM1L 
Gene Synonyms/Alias
 KIAA0851 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
250IVHYNGSKASFVQTRubiquitination[1]
273QRPNLKYKPLPQISKubiquitination[1]
423GVLHVGQKLEEQDEFubiquitination[1]
447DNANACAKQYAGTGAubiquitination[1]
456YAGTGALKTDFTRTGacetylation[2]
456YAGTGALKTDFTRTGubiquitination[1]
483NSMIRYYKNNFSDGFubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2 (By similarity). 
Sequence Annotation
 DOMAIN 122 451 SAC.
 MOD_RES 456 456 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Endoplasmic reticulum; Hydrolase; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 587 AA 
Protein Sequence
MATAAYEQLK LHITPEKFYV EACDDGADDV LTIDRVSTEV TLAVKKDVPP SAVTRPIFGI 60
LGTIHLVAGN YLIVITKKIK VGEFFSHVVW KATDFDVLSY KKTMLHLTDI QLQDNKTFLA 120
MLNHVLNVDG FYFSTTYDLT HTLQRLSNTS PEFQEMSLLE RADQRFVWNG HLLRELSAQP 180
EVHRFALPVL HGFITMHSCS INGKYFDWIL ISRRSCFRAG VRYYVRGIDS EGHAANFVET 240
EQIVHYNGSK ASFVQTRGSI PVFWSQRPNL KYKPLPQISK VANHMDGFQR HFDSQVIIYG 300
KQVIINLINQ KGSEKPLEQT FATMVSSLGS GMMRYIAFDF HKECKNMRWD RLSILLDQVA 360
EMQDELSYFL VDSAGQVVAN QEGVFRSNCM DCLDRTNVIQ SLLARRSLQA QLQRLGVLHV 420
GQKLEEQDEF EKIYKNAWAD NANACAKQYA GTGALKTDFT RTGKRTHLGL IMDGWNSMIR 480
YYKNNFSDGF RQDSIDLFLG NYSVDELESH SPLSVPRDWK FLALPIIMVV AFSMCIICLL 540
MAGDTWTETL AYVLFWGVAS IGTFFIILYN GKDFVDAPRL VQKEKID 587 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0030176; C:integral to endoplasmic reticulum membrane; IEA:Compara.
 GO:0016791; F:phosphatase activity; ISS:UniProtKB.
 GO:0034593; F:phosphatidylinositol bisphosphate phosphatase activity; IEA:Compara.
 GO:0004438; F:phosphatidylinositol-3-phosphatase activity; IEA:Compara.
 GO:0043812; F:phosphatidylinositol-4-phosphate phosphatase activity; IEA:Compara.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0046856; P:phosphatidylinositol dephosphorylation; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR002013; Syja_N. 
Pfam
 PF02383; Syja_N 
SMART
  
PROSITE
 PS50275; SAC 
PRINTS