CPLM-007829

Genbank Nucleotide ID

Lys-63-specific deubiquitinase BRCC36

Protein Synonyms/Alias

BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36

Mus musculus (Mouse)

Position	Peptide	Type	References
51	DDIRSDSKFTYTGTE	acetylation	[1]
51	DDIRSDSKFTYTGTE	ubiquitination	[2]
180	FQSIQAQKSSEYERI	ubiquitination	[2, 3, 4]

[1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144]

Functional Description

Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (By similarity).

DOMAIN 7 148 MPN.
MOTIF 122 135 JAMM motif.
METAL 122 122 Zinc; catalytic (By similarity).
METAL 124 124 Zinc; catalytic (By similarity).
METAL 135 135 Zinc; catalytic (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).

Acetylation; Alternative splicing; Chromatin regulator; Complete proteome; DNA damage; DNA repair; Hydrolase; Metal-binding; Metalloprotease; Nucleus; Protease; Reference proteome; Ubl conjugation pathway; Zinc.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0070531; C:BRCA1-A complex; ISS:UniProtKB.
GO:0070552; C:BRISC complex; ISS:UniProtKB.
GO:0000152; C:nuclear ubiquitin ligase complex; IEA:Compara.
GO:0030234; F:enzyme regulator activity; IEA:Compara.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0008237; F:metallopeptidase activity; ISS:UniProtKB.
GO:0031593; F:polyubiquitin binding; ISS:UniProtKB.
GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
GO:0006302; P:double-strand break repair; ISS:UniProtKB.
GO:0031572; P:G2 DNA damage checkpoint; ISS:UniProtKB.
GO:0070537; P:histone H2A K63-linked deubiquitination; ISS:UniProtKB.
GO:0045739; P:positive regulation of DNA repair; ISS:UniProtKB.
GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
GO:0010165; P:response to X-ray; IEA:Compara.

IPR000555; JAB1_Mov34_MPN_PAD1.