UniProt Accession

 RMLD_ECOLI; P37760; P76377 

Genbank Protein ID

 U09876; U00096; AP009048 

Genbank Nucleotide ID

 AAB88399.1; AAC75101.1; BAA15882.1 

Protein Name

 dTDP-4-dehydrorhamnose reductase 

Protein Synonyms/Alias

 dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase 

Gene Name

 rfbD 

Gene Synonyms/Alias

 rmlD; b2040; JW2025 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
69	AAHTAVDKAESEPEF	acetylation	[1]
137	ETKLAGEKALQEYCA	acetylation	[1]
145	ALQEYCAKHLIFRTS	acetylation	[1]
158	TSWVYAGKGNNFAKT	acetylation	[1]
164	GKGNNFAKTMLRLAK	acetylation	[1]
245	GIPLALNKLNAVPTT	acetylation	[1]
245	GIPLALNKLNAVPTT	pupylation	[2]
287	PDWQVGVKRMLNELF	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 

Functional Description

 Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well (By similarity). 

Sequence Annotation

 NP_BIND 7 11 NAD (By similarity).
 NP_BIND 11 12 NADP (By similarity).
 NP_BIND 30 31 NAD (By similarity).
 NP_BIND 39 40 NAD/NADP (By similarity).
 NP_BIND 62 65 NAD (By similarity).
 NP_BIND 63 65 NADP (By similarity).
 NP_BIND 128 132 NAD/NADP (By similarity).
 REGION 104 105 Substrate binding (By similarity).
 BINDING 12 12 NAD; via amide nitrogen (By similarity).
 BINDING 102 102 NADP; via carbonyl oxygen (By
 BINDING 153 153 Substrate; via amide nitrogen (By
 BINDING 154 154 NAD; via amide nitrogen (By similarity).
 BINDING 223 223 Substrate (By similarity).
 BINDING 260 260 Substrate (By similarity).  

Keyword

 Complete proteome; Lipopolysaccharide biosynthesis; Magnesium; Metal-binding; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0008831; F:dTDP-4-dehydrorhamnose reductase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019305; P:dTDP-rhamnose biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0045226; P:extracellular polysaccharide biosynthetic process; ISS:UniProtKB.
 GO:0009103; P:lipopolysaccharide biosynthetic process; ISS:UniProtKB.
 GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR005913; dTDP_dehydrorham_reduct.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF04321; RmlD_sub_bind 

SMART

  

PROSITE

  

PRINTS