CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001176
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sperm-associated antigen 7 
Protein Synonyms/Alias
  
Gene Name
 SPAG7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45QEQEKQQKVEFRKRMubiquitination[1]
67IQDSGQIKKKFQPMNubiquitination[2, 3]
166PASDYKDKYSHLIGKacetylation[4]
173KYSHLIGKGAAKDAAubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
  
Sequence Annotation
 DOMAIN 46 109 R3H.
 MOTIF 35 51 Nuclear localization signal (Potential).
 MOTIF 122 139 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 158 158 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 227 AA 
Protein Sequence
MADLLGSILS SMEKPPSLGD QETRRKAREQ AARLKKLQEQ EKQQKVEFRK RMEKEVSDFI 60
QDSGQIKKKF QPMNKIERSI LHDVVEVAGL TSFSFGEDDD CRYVMIFKKE FAPSDEELDS 120
YRRGEEWDPQ KAEEKRKLKE LAQRQEEEAA QQGPVVVSPA SDYKDKYSHL IGKGAAKDAA 180
HMLQANKTYG CVPVANKRDT RSIEEAMNEI RAKKRLRQSG EELPPTS 227 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR001374; R3H_ss-bd.
 IPR017330; Sperm-assoc_antigen_PAG7. 
Pfam
 PF01424; R3H 
SMART
 SM00393; R3H 
PROSITE
 PS51061; R3H 
PRINTS