UniProt Accession

 ICE1_ARATH; Q9LSE2; Q0WR77; Q2V3S3; Q5PNR8 

Genbank Protein ID

 AY195621; AB026648; AB016889; CP002686; CP002686; CP002686; AY079016; BT020379; AK228443 

Genbank Nucleotide ID

 AAP14668.1; BAB01738.1; BAB01738.1; AEE77208.1; AEE77209.1; AEE77210.1; AAL84972.1; AAV85734.1; BAF00372.1 

Protein Name

 Transcription factor ICE1 

Protein Synonyms/Alias

 Basic helix-loop-helix protein 116; AtbHLH116; bHLH 116; Inducer of CBF expression 1; Transcription factor EN 45; Transcription factor SCREAM; bHLH transcription factor bHLH116 

Gene Name

 SCRM 

Gene Synonyms/Alias

 BHLH116; EN45; ICE1; At3g26744; MDJ14.1; MLJ15.14 

Created Date

 July 27, 2013 

Organism

 Arabidopsis thaliana (Mouse-ear cress) 

NCBI Taxa ID

 3702 

Lysine Modification

Position	Peptide	Type	References
393	QTLSCRVKEELCPSS	sumoylation	[1, 2, 3]

Reference

 [1] SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis.
 Miura K, Jin JB, Lee J, Yoo CY, Stirm V, Miura T, Ashworth EN, Bressan RA, Yun DJ, Hasegawa PM.
 Plant Cell. 2007 Apr;19(4):1403-14. [PMID: 17416732]
 [2] ICE1 Ser403 is necessary for protein stabilization and regulation of cold signaling and tolerance.
 Miura K, Ohta M, Nakazawa M, Ono M, Hasegawa PM.
 Plant J. 2011 Jul;67(2):269-79. [PMID: 21447070]
 [3] SUMO, a heavyweight player in plant abiotic stress responses.
 Castro PH, Tavares RM, Bejarano ER, Azevedo H.
 Cell Mol Life Sci. 2012 Oct;69(19):3269-83. [PMID: 22903295] 

Functional Description

 Transcriptional activator that regulates the cold- induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. 

Sequence Annotation

 DOMAIN 303 352 bHLH.
 CROSSLNK 393 393 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Activator; Alternative splicing; Coiled coil; Complete proteome; Developmental protein; DNA-binding; Isopeptide bond; Nucleus; Reference proteome; Stress response; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 494 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:TAIR.
 GO:0003677; F:DNA binding; IDA:TAIR.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:TAIR.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:TAIR.
 GO:0050826; P:response to freezing; IMP:TAIR.
 GO:0010440; P:stomatal lineage progression; IMP:TAIR.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011598; bHLH_dom. 

Pfam

 PF00010; HLH 

SMART

 SM00353; HLH 

PROSITE

 PS50888; BHLH 

PRINTS