UniProt Accession

 HS3S1_HUMAN; O14792; B3KUA6; Q6PEY8 

Genbank Protein ID

 AF019386; AK096823; CH471069; BC057803 

Genbank Nucleotide ID

 AAB84388.1; BAG53368.1; EAW92699.1; AAH57803.1 

Protein Name

 Heparan sulfate glucosamine 3-O-sulfotransferase 1 

Protein Synonyms/Alias

 Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1 

Gene Name

 HS3ST1 

Gene Synonyms/Alias

 3OST; 3OST1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	GQQELLRKAGTLQDD	ubiquitination	[1]
128	PAYFTSPKVPERVYS	ubiquitination	[2]
169	YNHMQKHKPYPSIEE	ubiquitination	[2]
189	GRLNVDYKALNRSLY	ubiquitination	[1, 2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. 

Sequence Annotation

 NP_BIND 64 68 PAPS.
 NP_BIND 270 274 PAPS.
 BINDING 147 147 PAPS.
 BINDING 155 155 PAPS.
 BINDING 255 255 PAPS.
 CARBOHYD 48 48 N-linked (GlcNAc...) (Potential).
 CARBOHYD 192 192 N-linked (GlcNAc...) (Potential).
 CARBOHYD 242 242 N-linked (GlcNAc...) (Potential).
 CARBOHYD 249 249 N-linked (GlcNAc...) (Potential).
 DISULFID 256 265  

Keyword

 3D-structure; Complete proteome; Disulfide bond; Glycoprotein; Golgi apparatus; Polymorphism; Reference proteome; Signal; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 307 AA 

Protein Sequence

Gene Ontology

 GO:0005796; C:Golgi lumen; TAS:Reactome.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0008467; F:[heparan sulfate]-glucosamine 3-sulfotransferase 1 activity; IEA:EC.
 GO:0008146; F:sulfotransferase activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0006024; P:glycosaminoglycan biosynthetic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR000863; Sulfotransferase_dom. 

Pfam

 PF00685; Sulfotransfer_1 

SMART

  

PROSITE

  

PRINTS