| Tag | Content |
|---|
| CPLM ID | CPLM-025134 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | tRNA-splicing ligase RtcB homolog |
Protein Synonyms/Alias | |
Gene Name | D10Wsu52e |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 67 | GGFLPAMKQIGNVAA | ubiquitination | [1] | | 279 | DALVAMEKAMKRDKI | ubiquitination | [1] | | 285 | EKAMKRDKIIVNDRQ | ubiquitination | [1] | | 366 | EQHVVDGKERTLLVH | ubiquitination | [1] | | 465 | AIRVASPKLVMEEAP | ubiquitination | [1] | | 492 | CHDAGISKKAIKLRP | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'- phosphodiester. May act as a RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity). |
Sequence Annotation | METAL 122 122 Zinc (By similarity).
METAL 227 227 Zinc (By similarity).
METAL 259 259 Zinc (By similarity). |
Keyword | ATP-binding; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; tRNA processing; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 505 AA |
Protein Sequence | MSRNYNDELQ FLDKINKNCW RIKKGFVPNM QVEGVFYVND ALEKLMFEEL RNACRGGGVG 60
GFLPAMKQIG NVAALPGIVH RSIGLPDVHS GYGFAIGNMA AFDMNDPEAV VSPGGVGFDI 120
NCGVRLLRTN LDESDVQPVK EQLAQAMFDH IPVGVGSKGV ITMNAKDLEE ALEMGVDWSL 180
REGYAWAEDK EHCEEYGRML QADPNKVSPR AKKRGLPQLG TLGAGNHYAE IQVVDEIFNE 240
YAAKKMGIDH KGQVCVMIHS GSRGLGHQVA TDALVAMEKA MKRDKIIVND RQLACARIAS 300
PEGQDYLKGM AAAGNYAWVN RSSMTFLTRQ AFAKVFNTTP DDLDLHVIYD VSHNIAKVEQ 360
HVVDGKERTL LVHRKGSTRA FPPHHPLIAV DYQLTGQPVL IGGTMGTCSY VLTGTEQGMT 420
ETFGTTCHGA GRALSRAKSR RNLDFQDVLD KLADMGIAIR VASPKLVMEE APESYKNVTD 480
VVNTCHDAGI SKKAIKLRPI AVIKG 505 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:MGI. GO:0072669; C:tRNA-splicing ligase complex; IEA:HAMAP. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0003972; F:RNA ligase (ATP) activity; IEA:HAMAP. GO:0001701; P:in utero embryonic development; IMP:MGI. GO:0001890; P:placenta development; IMP:MGI. GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:HAMAP. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |