UniProt Accession

 YOEB_ECOLI; P69348; P56605; Q2EES4; Q2MAY7 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 ABD18681.1; BAE76569.1 

Protein Name

 Toxin YoeB 

Protein Synonyms/Alias

 Putative endoribonuclease YoeB; Putative mRNA interferase Yoeb 

Gene Name

 yoeB 

Gene Synonyms/Alias

 b4539; JW5331 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
32	KKINELIKDTRRTPF	acetylation	[1]
42	RRTPFEGKGKPEPLK	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Toxic component of a toxin-antitoxin (TA) module. Its mode of function is controversial; it has been proposed to be an mRNA interferase but also an inhibitor of translation initiation. When overproduced in wild-type cells, inhibits bacterial growth and translation by cleavage of mRNA molecules while it has a weak effect on colony forming ability. Overproduction of Lon protease specifically activates YoeB-dependent mRNA cleavage, leading to lethality. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor. 

Sequence Annotation

 ACT_SITE 46 46 Proton acceptor (Probable).
 ACT_SITE 83 83 Proton donor (Probable).  

Keyword

 3D-structure; Complete proteome; Endonuclease; Hydrolase; Nuclease; Reference proteome; Repressor; RNA-binding; Toxin; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 84 AA 

Protein Sequence

Gene Ontology

 GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0006379; P:mRNA cleavage; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006401; P:RNA catabolic process; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR009614; Addiction_module_toxin_Txe/Yoe. 

Pfam

 PF06769; Plasmid_Txe 

SMART

  

PROSITE

  

PRINTS