CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015119
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 4 gamma 1 
Protein Synonyms/Alias
 eIF-4-gamma 1; eIF-4G 1; eIF-4G1 
Gene Name
 Eif4g1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
197RDPNQGGKDITEEIMubiquitination[1]
528KIKELNKKEAVGDLLubiquitination[1]
597NIQPGEQKYEYKSDQubiquitination[1]
601GEQKYEYKSDQWKPLubiquitination[1]
606EYKSDQWKPLNLEEKubiquitination[1]
774RVRSILNKLTPQMFQubiquitination[1]
799IDTEERLKGVIDLIFubiquitination[1]
885AEERGRLKEELEEARubiquitination[1]
904RRSLGNIKFIGELFKubiquitination[1]
911KFIGELFKLKMLTEAubiquitination[1]
954GKDLDFAKAKPRMDQubiquitination[1]
956DLDFAKAKPRMDQYFacetylation[2]
1078SRLTKITKPGSIDSNubiquitination[1]
1449ELRRQLEKLLKDGGSacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (By similarity). 
Sequence Annotation
 DOMAIN 765 993 MIF4G.
 DOMAIN 1241 1363 MI.
 DOMAIN 1429 1599 W2.
 REGION 179 207 PABPC1-binding (By similarity).
 REGION 611 622 EIF4E-binding (By similarity).
 REGION 686 1089 eIF3/EIF4A-binding (By similarity).
 REGION 1450 1600 EIF4A-binding (By similarity).
 REGION 1585 1600 Necessary but not sufficient for MKNK1-
 MOD_RES 212 212 Phosphothreonine.
 MOD_RES 214 214 Phosphothreonine.
 MOD_RES 651 651 Phosphothreonine (By similarity).
 MOD_RES 1032 1032 Phosphoserine (By similarity).
 MOD_RES 1096 1096 Phosphoserine (By similarity).
 MOD_RES 1099 1099 N6-acetyllysine (By similarity).
 MOD_RES 1147 1147 Phosphoserine (By similarity).
 MOD_RES 1149 1149 Phosphoserine (By similarity).
 MOD_RES 1187 1187 Phosphoserine; by PKC/PRKCA (By
 MOD_RES 1189 1189 Phosphoserine (By similarity).
 MOD_RES 1211 1211 Phosphoserine.
 MOD_RES 1213 1213 Phosphothreonine (By similarity).
 MOD_RES 1231 1231 Phosphoserine.
 MOD_RES 1597 1597 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1600 AA 
Protein Sequence
MNKAPQPTGP PPARSPGLPQ PAFPPGQTAP VVFSTPQATQ MNTPSQPRQG GFRSLQHFYP 60
SRAQPPSSAA SRVQSAAPAR PGPAPHVYPA GSQVMMIPSQ ISYSASQGAY YIPGQGRSTY 120
VVPTQQYPVQ PGAPGFYPGA SPTEFGTYAG AYYPAQGVQQ FPASVAPAPV LMNQPPQIAP 180
KRERKTIRIR DPNQGGKDIT EEIMSGARTA STPTPPQTGG SLEPQPNGES PQVAVIIRPD 240
DRSQGAAIGG RPGLPGPEHS PGTESQPSSP SPTPSPPPIL EPGSESNLGV LSIPGDTMTT 300
GMIPMSVEES TPISCETGEP YCLSPEPTLA EPILEVEVTL SKPIPESEFS SSPLQVSTAL 360
VPHKVETHEP NGVIPSEDLE PEVESSTEPA PPPLSPCASE SLVPIAPTAQ PEELLNGAPS 420
PPAVDLSPVS EPEEQAKKVS SAALASILSP APPVAPSDTS PAQEEEMEED DDDEEGGEAE 480
SEKGGEDVPL DSTPVPAQLS QNLEVAAATQ VAVSVPKRRR KIKELNKKEA VGDLLDAFKE 540
VDPAVPEVEN QPPTGSNPSP ESEGSMVPTQ PEETEETWDS KEDKIHNAEN IQPGEQKYEY 600
KSDQWKPLNL EEKKRYDREF LLGFQFIFAS MQKPEGLPHI TDVVLDKANK TPLRQLDPSR 660
LPGINCGPDF TPSFANLGRP ALSNRGPPRG GPGGELPRGP AGLGPRRSQQ GPRKETRKII 720
SSVIMTEDIK LNKAEKAWKP SSKRTAADKD RGEEDADGSK TQDLFRRVRS ILNKLTPQMF 780
QQLMKQVTQL AIDTEERLKG VIDLIFEKAI SEPNFSVAYA NMCRCLMALK VPTTEKPTVT 840
VNFRKLLLNR CQKEFEKDKD DDEVFEKKQK EMDEAATAEE RGRLKEELEE ARDIARRRSL 900
GNIKFIGELF KLKMLTEAIM HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFAKAKPRMD 960
QYFNQMEKII KEKKTSSRIR FMLQDVLDLR QSNWVPRRGD QGPKTIDQIH KEAEMEEHRE 1020
HIKVQQLMAK GSDKRRGGPP GPPINRGLPL VDDGGWNTVP ISKGSRPIDT SRLTKITKPG 1080
SIDSNNQLFA PGGRLSWGKG SSGGSGAKPS DTASEATRPA TLNRFSALQQ TLPAENTDNR 1140
RVVQRSSLSR ERGEKAGDRG DRLERSERGG DRGDRLDRAR TPATKRSFSK EVEERSRERP 1200
SQPEGLRKAA SLTEDRGRDP VKREATLPPV SPPKAALSVD EVEKKSKAII EEYLHLNDMK 1260
EAVQCVQELA SPSLLFIFVR LGIESTLERS TIAREHMGRL LHQLLCAGHL STAQYYQGLY 1320
ETLELAEDME IDIPHVWLYL AELITPILQE DGVPMGELFR EITKPLRPMG KATSLLLEIL 1380
GLLCKSMGPK KVGMLWREAG LSWREFLAEG QDVGSFVAEK KVEYTLGEES EAPGQRTLAF 1440
EELRRQLEKL LKDGGSNQRV FDWIDANLNE QQIASNTLVR ALMTTVCYSA IIFETPLRVD 1500
VQVLKVRARL LQKYLCDEQK ELQALYALQA LVVTLEQPAN LLRMFFDALY DEDVVKEDAF 1560
YSWESSKDPA EQQGKGVALK SVTAFFNWLR EAEDEESDHN 1600 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR003891; Initiation_fac_eIF4g_MI.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3.
 IPR003307; W2_domain. 
Pfam
 PF02847; MA3
 PF02854; MIF4G
 PF02020; W2 
SMART
 SM00515; eIF5C
 SM00544; MA3
 SM00543; MIF4G 
PROSITE
 PS51366; MI
 PS51363; W2 
PRINTS