UniProt Accession

 YAP2_YEAST; P24813; D6VT53; Q02259 

Genbank Protein ID

 L14289; X69106; S68847; U33007; M58331; BK006938 

Genbank Nucleotide ID

 AAA02920.1; CAA48858.1; AAB29937.1; AAB64878.1; AAA34463.1; DAA12263.1 

Protein Name

 AP-1-like transcription activator YAP2 

Protein Synonyms/Alias

 Cadmium resistance protein 1; Transcription factor CAD1 

Gene Name

 CAD1 

Gene Synonyms/Alias

 YAP2; YDR423C; D9461.12 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
105	SLLSEITKYRAKNSD	acetylation	[1]
250	SERLTAEKIDTSACQ	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Transcription activator closely related to YAP1. Like YAP1, its activity can be induced by cadmium and peroxide stress, probably involving similar mechanisms inducing nuclear accumulation and thus transcription activation. YAP2, like YAP1, preferentially binds to promoters with the core binding site 5'- TTA[CG]TAA-3', but does not activate transcription of genes known to be activated by YAP1 in response to oxidative stress (such as TRX2, SSA1, GSH1). It is still not clear whether it is involved in oxidative stress response. When overexpressed, it confers pleiotropic drug-resistance and increases cellular tolerance to cadmium, iron chelators and zinc. 

Sequence Annotation

 DOMAIN 43 106 bZIP.
 REGION 46 69 Basic motif (By similarity).
 REGION 71 99 Leucine-zipper (By similarity).
 DISULFID 356 387 In nuclear retained form; alternate.
 DISULFID 356 378 In nuclear retained form; alternate.
 DISULFID 378 387 In nuclear retained form; alternate.  

Keyword

 Activator; Cadmium resistance; Complete proteome; Cytoplasm; Disulfide bond; DNA-binding; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 409 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:SGD.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0071276; P:cellular response to cadmium ion; IDA:SGD.
 GO:0036003; P:positive regulation of transcription from RNA polymerase II promoter in response to stress; IGI:SGD. 

Interpro

 IPR004827; bZIP.
 IPR013910; TF_PAP1.
 IPR023167; Yap1_redox_dom. 

Pfam

 PF00170; bZIP_1
 PF08601; PAP1 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS