UniProt Accession

 EFP_ECOLI; P0A6N4; P33398; Q2M6F7 

Genbank Protein ID

 X61676; U14003; U00096; AP009048 

Genbank Nucleotide ID

 CAA43851.1; AAA97046.1; AAC77107.1; BAE78149.1 

Protein Name

 Elongation factor P 

Protein Synonyms/Alias

 EF-P 

Gene Name

 efp 

Gene Synonyms/Alias

 b4147; JW4107 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
31	VEASEFVKPGKGQAF	acetylation	[1]
54	LTGTRVEKTFKSTDS	acetylation	[1]
175	VQIGEVIKVDTRSGE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Beta-lysylation at Lys-34 is required for alleviation. The Pro codons and their context do not affect activity; only consecutive Pro residues (not another amino acid) are affected by EF-P. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis. 

Sequence Annotation

 MOD_RES 34 34 N6-(3,6-diaminohexanoyl)-5-hydroxylysine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Hydroxylation; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 188 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0043022; F:ribosome binding; IDA:EcoCyc.
 GO:0003746; F:translation elongation factor activity; IDA:EcoCyc.
 GO:0043043; P:peptide biosynthetic process; IEA:InterPro. 

Interpro

 IPR015365; Elong-fact-P_C.
 IPR012340; NA-bd_OB-fold.
 IPR014722; Rib_L2_dom2.
 IPR020599; Transl_elong_fac_P/YeiP.
 IPR013185; Transl_elong_KOW-like.
 IPR001059; Transl_elong_P/YeiP_cen.
 IPR013852; Transl_elong_P/YeiP_CS.
 IPR011768; Transl_elongation_fac_P.
 IPR008991; Translation_prot_SH3-like. 

Pfam

 PF01132; EFP
 PF08207; EFP_N
 PF09285; Elong-fact-P_C 

SMART

 SM00841; Elong-fact-P_C 

PROSITE

 PS01275; EFP 

PRINTS