UniProt Accession

 ASH1_YEAST; P34233; D6VX15 

Genbank Protein ID

 X74151; Z28185; M88605; BK006944 

Genbank Nucleotide ID

 CAA52253.1; CAA82028.1; AAA34830.1; DAA08981.1 

Protein Name

 Transcriptional regulatory protein ASH1 

Protein Synonyms/Alias

 Daughter cells HO repressor protein 

Gene Name

 ASH1 

Gene Synonyms/Alias

 YKL185W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
272	IPSILEAKDQFKDLS	ubiquitination	[1]
338	EKLVQEVKHQRQLRG	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth. 

Sequence Annotation

 ZN_FING 499 526 GATA-type; atypical.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 465 465 Phosphoserine.  

Keyword

 Chromatin regulator; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 588 AA 

Protein Sequence

Gene Ontology

 GO:0005933; C:cellular bud; IDA:SGD.
 GO:0033698; C:Rpd3L complex; IDA:SGD.
 GO:0070210; C:Rpd3L-Expanded complex; IDA:SGD.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IDA:SGD.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:1900477; P:negative regulation of G1/S transition of mitotic cell cycle by negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900474; P:negative regulation of mating type switching by negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900461; P:positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD. 

Interpro

 IPR000679; Znf_GATA.
 IPR013088; Znf_NHR/GATA. 

Pfam

 PF00320; GATA 

SMART

 SM00401; ZnF_GATA 

PROSITE

 PS00344; GATA_ZN_FINGER_1
 PS50114; GATA_ZN_FINGER_2 

PRINTS