CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042010
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol 4-kinase type 2 beta 
Protein Synonyms/Alias
 Phosphatidylinositol 4-kinase type 2-beta 
Gene Name
 PI4K2B 
Gene Synonyms/Alias
 hCG_19614 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
41SSGSYFVKDPKRKIIubiquitination[1, 2, 3, 4, 5]
44SYFVKDPKRKIIGVFubiquitination[5]
46FVKDPKRKIIGVFKPubiquitination[4]
54IIGVFKPKSEEPYGQubiquitination[4, 5]
65PYGQLNPKWTKYVHKubiquitination[1, 4, 6]
68QLNPKWTKYVHKVCCubiquitination[5]
72KWTKYVHKVCCPCCFubiquitination[4, 5]
102GAYLVDNKLHLSIVPubiquitination[4]
140GKKYALEKVPKVGRKubiquitination[4, 5]
176EAEYWLRKFEADPLPubiquitination[4, 5, 6]
188PLPENIRKQFQSQFEubiquitination[5]
256NGLAFPFKHPDEWRAubiquitination[1, 4, 5, 6]
315KTDKGFDKATFESQMubiquitination[1, 5, 6]
340TQALRDGKSPFQLVQubiquitination[4, 5]
379TQTVNCRKPFFSSW*ubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 385 AA 
Protein Sequence
MNAFLDDPEF ADIMLRAEQA IEVGIFPERI SQGSSGSYFV KDPKRKIIGV FKPKSEEPYG 60
QLNPKWTKYV HKVCCPCCFG RGCLIPNQGY LSEAGAYLVD NKLHLSIVPK TKVVWLVSET 120
FNYNAIDRAK SRGKKYALEK VPKVGRKFHR IGLPPKIGSF QLFVEGYKEA EYWLRKFEAD 180
PLPENIRKQF QSQFERLVIL DYIIRNTDRG NDNWLVRYEK QKCEKEIDHK ESKWIDDEEF 240
LIKIAAIDNG LAFPFKHPDE WRAYPFHWAW LPQAKVPFSE EIRNLILPYI SDMNFVQDLC 300
EDLYELFKTD KGFDKATFES QMSVMRGQIL NLTQALRDGK SPFQLVQIPC VIVERSQGGS 360
QGRIVHLSNS FTQTVNCRKP FFSSW 385 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro. 
Interpro
 IPR000403; PI3/4_kinase_cat_dom. 
Pfam
 PF00454; PI3_PI4_kinase 
SMART
  
PROSITE
  
PRINTS