UniProt Accession

 RAI3_HUMAN; Q8NFJ5; B3KV45; O95357 

Genbank Protein ID

 AF095448; AF506289; AK001761; AK122672; CH471094; BC003665 

Genbank Nucleotide ID

 AAC98506.1; AAM77594.1; BAA91890.1; BAG53657.1; EAW96289.1; AAH03665.1 

Protein Name

 Retinoic acid-induced protein 3 

Protein Synonyms/Alias

 G-protein coupled receptor family C group 5 member A; Orphan G-protein-coupling receptor PEIG-1; Retinoic acid-induced gene 1 protein; RAIG-1 

Gene Name

 GPRC5A 

Gene Synonyms/Alias

 GPCR5A; RAI3; RAIG1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
285	PVEDAFCKPQLVKKS	ubiquitination	[1, 2, 3]
290	FCKPQLVKKSYGVEN	ubiquitination	[1]
333	LQNQPPQKEFSIPRA	ubiquitination	[1, 3, 4]
348	HAWPSPYKDYEVKKE	ubiquitination	[1, 2, 3, 4, 5, 6]
353	PYKDYEVKKEGS***	ubiquitination	[1, 4, 5, 6]

Reference

 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Unknown. This G-protein coupled receptor could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. The comparable expression level in fetal lung and kidney with adult tissues suggests a possible role in embryonic development and maturation of these organs. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. 

Sequence Annotation

 MOD_RES 301 301 Phosphoserine.
 MOD_RES 345 345 Phosphoserine.
 CARBOHYD 158 158 N-linked (GlcNAc...) (Potential).  

Keyword

 Cell membrane; Complete proteome; Cytoplasmic vesicle; G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Transducer; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 357 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0004930; F:G-protein coupled receptor activity; TAS:ProtInc. 

Interpro

 IPR017978; GPCR_3_C. 

Pfam

 PF00003; 7tm_3 

SMART

  

PROSITE

 PS00979; G_PROTEIN_RECEP_F3_1
 PS00980; G_PROTEIN_RECEP_F3_2
 PS00981; G_PROTEIN_RECEP_F3_3
 PS50259; G_PROTEIN_RECEP_F3_4 

PRINTS