CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011376
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 
Protein Synonyms/Alias
 D-fructose-6-phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; GFAT 1; GFAT1; Hexosephosphate aminotransferase 1 
Gene Name
 GFPT1 
Gene Synonyms/Alias
 GFAT; GFPT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
139LKKFLESKGYDFESEubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. 
Sequence Annotation
 DOMAIN 2 305 Glutamine amidotransferase type-2.
 DOMAIN 377 516 SIS 1.
 DOMAIN 548 689 SIS 2.
 REGION 313 680 Isomerase.
 REGION 394 395 Substrate-binding.
 REGION 439 441 Substrate-binding.
 ACT_SITE 2 2 For GATase activity (By similarity).
 BINDING 444 444 Substrate.
 BINDING 584 584 Substrate.
 MOD_RES 261 261 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Aminotransferase; Complete proteome; Congenital myasthenic syndrome; Disease mutation; Glutamine amidotransferase; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 699 AA 
Protein Sequence
MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GFDGGNDKDW EANACKIQLI 60
KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG EPSPVNSHPQ RSDKNNEFIV 120
IHNGIITNYK DLKKFLESKG YDFESETDTE TIAKLVKYMY DNRESQDTSF TTLVERVIQQ 180
LEGAFALVFK SVHFPGQAVG TRRGSPLLIG VRSEHKLSTD HIPILYRTGK DKKGSCNLSR 240
VDSTTCLFPV EEKAVEYYFA SDASAVIEHT NRVIFLEDDD VAAVVDGRLS IHRIKRTAGD 300
HPGRAVQTLQ MELQQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT VNLGGLKDHI 360
KEIQRCRRLI LIACGTSYHA GVATRQVLEE LTELPVMVEL ASDFLDRNTP VFRDDVCFFL 420
SQSGETADTL MGLRYCKERG ALTVGITNTV GSSISRETDC GVHINAGPEI GVASTKAYTS 480
QFVSLVMFAL MMCDDRISMQ ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV 540
LIMGRGYHYA TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA 600
KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV IPLQLLAFHL 660
AVLRGYDVDF PRNLAKSVTV E 681 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; TAS:ProtInc.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:ProtInc.
 GO:0006002; P:fructose 6-phosphate metabolic process; TAS:ProtInc.
 GO:0006042; P:glucosamine biosynthetic process; IEA:Compara.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0045719; P:negative regulation of glycogen biosynthetic process; IEA:Compara.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0009744; P:response to sucrose stimulus; IEA:Compara.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; TAS:Reactome. 
Interpro
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR005855; GlmS_trans.
 IPR001347; SIS. 
Pfam
 PF00310; GATase_2
 PF01380; SIS 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2
 PS51464; SIS 
PRINTS