CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002958
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thymidylate kinase 
Protein Synonyms/Alias
 Thymidine monophosphate kinase; dTMP kinase; TMPK 
Gene Name
 tmk 
Gene Synonyms/Alias
 ycfG; b1098; JW1084 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
4****MRSKYIVIEGLacetylation[1]
49GGTQLAEKLRSLVLDacetylation[1]
209TTVTHWVKELDA***acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the reversible phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), using ATP as its preferred phosphoryl donor. Situated at the junction of both de novo and salvage pathways of deoxythymidine triphosphate (dTTP) synthesis, is essential for DNA synthesis and cellular growth. 
Sequence Annotation
 NP_BIND 10 17 ATP.
 REGION 147 159 LID.
 BINDING 12 12 TMP.
 BINDING 74 74 TMP.
 BINDING 78 78 TMP.
 BINDING 100 100 TMP.
 BINDING 105 105 TMP.
 BINDING 108 108 TMP.
 BINDING 109 109 TMP.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Kinase; Nucleotide biosynthesis; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 213 AA 
Protein Sequence
MRSKYIVIEG LEGAGKTTAR NVVVETLEQL GIRDMVFTRE PGGTQLAEKL RSLVLDIKSV 60
GDEVITDKAE VLMFYAARVQ LVETVIKPAL ANGTWVIGDR HDLSTQAYQG GGRGIDQHML 120
ATLRDAVLGD FRPDLTLYLD VTPEVGLKRA RARGELDRIE QESFDFFNRT RARYLELAAQ 180
DKSIHTIDAT QPLEAVMDAI RTTVTHWVKE LDA 213 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004798; F:thymidylate kinase activity; IDA:EcoCyc.
 GO:0006233; P:dTDP biosynthetic process; IDA:EcoliWiki.
 GO:0006235; P:dTTP biosynthetic process; IDA:EcoliWiki.
 GO:0015949; P:nucleobase-containing small molecule interconversion; IDA:EcoliWiki. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR018095; Thymidylate_kin_CS.
 IPR018094; Thymidylate_kinase. 
Pfam
  
SMART
  
PROSITE
 PS01331; THYMIDYLATE_KINASE 
PRINTS